GC/FID
In GC/FID, the FID or flame ionization detector detects analytes by measuring an electrical current generated by electrons from burning carbon particles in the sample.
The flame ionization detector (FID) is a non-selective detector used in conjunction with gas chromatography. Because it is non-selective, there is a potential for many non-target compounds present in samples to interfere with this analysis and for poor resolution especially in complex samples. The FID works by directing the gas phase output from the column into a hydrogen flame. A voltage of 100-200V is applied between the flame and an electrode located away from the flame. The increased current due to electrons emitted by burning carbon particles is then measured. Although the signal current is very small (the ionization efficiency is only 0.0015%) the noise level is also very small (<10-13 amp) and with a well-optimized system, sensitivities of 5 x 10-12 g/ml for n-heptane at a signal/noise ratio of 2 can be easily realized. Except for a very few organic compounds (e.g. carbon monoxide, etc.) the FID detects all carbon containing compounds. The detector also has an extremely wide linear dynamic range that extends over, at least five orders of magnitude with a response index between 0.98-1.02.
Application Notes – GC/FID
Analysis of Ephedra Alkaloids by GC
Derivatized alkaloid compounds in banned herbal supplement Ephedra separated by capillary GC/FID in 4 minutes.
Separation of Para- and Meta-Xylene on Capillary GC Column
Para- and meta-xylene were more completely resolved on a Carbowax capillary column than on columns typically used for this determination.
A Diagnostic Tool for Smith-Lemli-Opitz Syndrome
Capillary GC/FID used to separate cholesterol and precursor molecule in plasma to identify genetic defect.
Decreasing GC Analysis Time with Small Diameter Columns
Shorter, smaller diameter GC capillary columns shown to decrease analysis time by 50% without loss of resolution for PAH\’s, phenols and lemon oil fingerprint compounds.
Polymer Degradation Mechanisms by Pyrolysis GC
Three mechanisms of polymer degradation by pyrolysis studied using pyrolysis GC unit.
2-Bromo-3-methyl butyric acid by GC Using Chiraldex B-TA Column
The enantiomers of the methyl ester of 2-bromo-3-methyl butyric acid were separated by GC using a Chiraldex B-TA column and detected by FID.
1,3-Butadiene diepoxide by GC Using Chiraldex G-PN Column
The enantiomers of 1,3-butadiene diepoxide were separated by GC using a Chiraldex G-PN column and detected by FID.
1,2,4-Butanetriol by GC Using Chiraldex G-BP Column
The enantiomers of the o-trifluoroacetyl derivative of 1,2,4-butanetriol were separated by GC using a Chiraldex G-BP column and detected by FID.
2-Bromo-3-methyl butyric acid by GC Using Chiraldex G-TA Column
The enantiomers of 2-bromo-3-methyl butyric acid were separated by GC using a Chiraldex G-TA column and detected by FID.
1,2,4-Butanetriol by GC Using Chiraldex G-PN Column
The enantiomers of the o-trifluoroacetyl derivative of 1,2,4-butanetriol were separated by GC using a Chiraldex G-PN column and detected by FID.
2-Butanol by GC Using Chiraldex G-PN Column
The enantiomers of the o-crifluoroacetyl derivative of 2-butanol were separated by GC using a Chiraldex G-PN Column and detected by FID.
2-Butanol by GC Using Chiraldex G-TA Column-1
The enantiomers of the o-trifluoroacetyl derivative of 2-butanol were separated by GC using a Chiraldex G-TA column and detected by FID.
2-Butanol by GC Using Chiraldex G-TA Column-2
The enantiomers of the p-toluenesulfonyl derivative of 2-butanol were separated by GC using a Chiraldex G-TA column and detected by FID.
2-Butanol by GC Using Chiraldex G-TA Column-3
The enantiomers of underivatized 2-butanol were separated by GC using a Chiraldex G-TA column and detected by FID.
2-Bromopentane by GC Using Chiraldex G-PN Column
The enantiomers of 2-bromopentane were separated by GC using a Chiraldex G-PN column and detected by FID.
2-Bromopentane by GC Using Chiraldex G-TA Column
The enantiomers of 2-bromopentane were separated by GC using a Chiraldex G-TA column and detected by FID.
Diacetone isomers by GC Using Chiraldex G-BP Column
The enantiomers of diacetone were separated by GC using a Chiraldex G-BP column and detected by FID.
Diacetone isomers by GC Using Chiraldex G-TA Column
The enantiomers of diacetone were separated by GC using a Chiraldex G-TA column and detected by FID.
2,5-Dimethoxy tetrahydrofuran by GC Using Chiraldex G-BP Column
The enantiomers of 2,5-dimethoxy tetrahydrofuran were separated by GC using a Chiraldex G-BP column and detected by FID.
2,5-Dimethoxy tetrahydrofuran by GC Using Chiraldex G-PN Column
The enantiomers of 2,5-dimethoxy tetrahydrofuran were separated by GC using a Chiraldex G-PN column and detected by FID.
More Application Notes from GC/FID