Topics - Waxes

Waxes There are two types of waxes associated with chromatography; there are the hydrocarbon waxes, usually white in color, that consist of mixtures of high molecular weight aliphatic hydrocarbons and may be soft or hard in texture; then there is bee wax which is a far more complex wax, yellow to brownish yellow in color, somewhat brittle in texture and often having a faint odor of honey. Bee wax consists of esters of even-numbered (ca C24 to C26), straight chained monohydric alcohols with straight chained (up to C36) even-numbered acids. (e.g. triacontanol hexadecanate) These esters are mixed with about 20%w/w odd numbered (C21 toC23) straight chained hydrocarbons. Waxes contain extensive quantities of aliphatic straight chains and are thus, strongly dispersive (hydrophobic) in interactive character. Consequently, hydrocarbon waxes have been successfully separated on poly[dimethylsiloxane] stationary phases by gas chromatography employing a 30 m capillary column 0.54 mm I.D. having a film thickness of 0.01 micron and programmed from 40C to 350C using helium as the carrier gas