Topics - Warfarin
Warfarin or Coumadin is a very strong anti-coagulant which functions by inhibiting vitamin K-dependent coagulation factors. It was originally used as a rat poison but has also found use in medicine in very dilute form as an anticoagulant for thrombosis, pulmonary embolism and cardiac valve repleacement. Chemically it is 3-(-acetonylbenzyl)-4-hydroxycoumarin and is supplied as a racemic mixture of the R and S-enantiomers. The Warfarin enantiomers can be separated and quantitatively determined by liquid chromatography using a 25 cm long 4.6 mm I.D. packed with a cyclodextrin stationary phase (CYCLOBOND 1) 5 micron particle size. The mobile phase is 90%v/v acetonitrile, 10 %v/v methanol 0.2%v/v acetic acid and 0.2%v/v triethylamine. Base-line separation is obtained for the two enantiomers.