Topics - Caryophylene

Caryophylene Caryophyllene is a sesquiterpinoid constituent of many essential oils, in particular, clove oil and in the stems and flowers of Syzyglum aromaticum. There are two forms caryophyllene a caryophyllene and B caryophyllene and they usually occur together with isocaryophyllene. Caryophyllene is a hydrocarbon with a molecular weight of 204.36 and elemental analysis shows it contains 88.16% carbon and 11.84% hydrogen. Caryophyllene is present in many essential oils, for example lime oil, and is frequently used in the blending of synthetic oils and fragrances. The analysis of lime oil, including the separation and estimation of the caryophyllene content, can easily be carried out by gas chromatography. A very effective stationary phase for this purpose is poly (5%diphenyl-95%-dimethylsiloxane). Although the diphenyl group will contribute some induced polaizability and be capable of interacting with polar and semi-polar solutes, it is largely a dispersive stationary phase. In addition, as caryophyllene is a hydrocarbon, the only retentive forces that will be active on this solute will be dispersive. The introduction of the diphenyl groups into the stationary phase increases its temperature stability An example of a suitable capillary column would be 30m long, 250 micron ID carrying a film of stationary phase 0.25 micron thick. At a linear mobile phase velocity of 25 cm/sec and temperature programmed form 155C to 200C the components of lime oil ranging from pinene to bisabolen can be eluted in about 30 minutes.