Topics - Peak Capacity
Peak capacity is a measure of the number of peaks that can be separated from one another up to a predefined capacity ratio (k’). An equation that gives a numerical value for the peak capacity can be developed from the Plate theory. The theory assumes that all the peaks are separated from one another by four standard deviations of the Gaussian peak profile. As the the capacity ratio of the peaks increase, the peaks get progressively broader, so, it is clear that the majority pf the peaks are contained in the early part of the chromatogram where the peaks are relatively narrow. The peak capacity gives an exaggerated value for the number of solutes that can be separated, as, in an actual separation, the eluted peaks do not line themselves up conveniently four standard deviations apart. Peak capacity is largely of theoretical interest, it does not help much in the practice of chromatography.