Topics - Band

Band The term band originated from the first chromatographic separation which was carried out by Tswett in the late 1890s. Tswett separated some plant pigments employing a liquid-solid phase system in a tubular column and the separated pigments formed different colored stripes across the diameter of the tube forming a strong contrast with the white adsorbent; these stripes were given the name bands. The individual pigments were recovered by extruding the adsorbent from the tube and slicing each band away from its neighbor with a knife. Each band was then extracted with a solvent. The term band persisted for some years and when elution peaks were obtained from the early gas chromatographs they were also sometimes termed solute bands. Today, the term band has largely fallen into disuse and is rarely used in modern column chromatography. The terminology, however, is still used thin layer chromatography and in electrophoresis.