Topics - Atenolol
Atenalol is a beta-selective (cardioselective) adrenoceptor or blocking agent. The drug slows the heart beat, reduces hypertension and consequently is used in cases of angina, hypertension and arrhythmias. The chemical name of Atenolol is 4-[2-hydroxy-3- [(1-methylethyl)amino] propoxy]benzeneacetamide; it has a molecular weight of 266.34 and elemental analysis shows it contains 63.14% carbon, 8.33% hydrogen, 10.52% nitrogen and 18.02% oxygen. Atenolol reacts directly with the fluorescence agent 4(N-chloroformethyl-N-methylamino-7-N-N-dimethylaminosulphonyl -2,1,2-benzoxydazole to form a fluorescent derivative as a 1-1 aduct with the secondary amino group of the B
blocker. The reaction proceeds readily with no catalyst. The derivatives can be separated on a reversed phase column 15 cm long and 4.6 mm in diameter packed with 5 micron particles and a water/methanol or water/acetonitrile mobile phase.