Ion Chromatography - The Separation of Some Monovalent and Divalent Cations

An example of a separation of some transmission metal standards and a plating bath electrolyte is shown in figure 31 The column was an Alltech Transition Metal Column 25 cm long and 4.6 mm in diameter packed with a silica based stationary phase (an octadecane bonded silica carrying about 20% carbon) with a particle diameter 7 mm.

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(Courtesy of Altech.)

Figure 31. Separation of Transition Metal Standards and those Contained in the Contents of a Plating Bath.

The mobile phase consisted of 2mM sodium octane sulphonate, 40mM Tartaric acid in 5% aqueous methanol adjusted to a pH of 4.0 with sodium hydroxide.

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(Courtesy of Altech)

Figure 32. Chromatogram of the Transition Metals in a Fermentation Broth

The octane sulphonate was absorbed onto the surface of the reverse phase producing an adsorbed ion exchanger. The chromatogram of the plating bath electrolyte was obtained by electrical conductivity detection whereas the chromatogram of the standards was obtained by employing a post column reaction system using 4-(2-pyridylazo) resorcinol as the derivatizing reagent in conjunction with a UV/vis detector operating at 520 nm. Another example of the use of the Alltech Transition Metal Column for the determination of the transition metals in a fermentation broth is shown in figure 32.