Analytes - caffeine

caffeine: CAS No. 58-08-02, C8H10N4O2, molecular weight 194.19, boiling point 178°C, melting point 238°C, density 1.23gm/mL, vapor pressure 15mm Hg at 89°C, water soluble is a white crystalline solid generally considered as a stimulant. It is widely used in medications and as a food additive. It is prepared by extraction from decaffeinated coffee. It is a central nervous system stimulant and a phosphodiesterase inhibitor. It is used in combination with aspirin to treat headaches and to treat asthma. Prolonged, high intake of it may produce tolerance, habituation, and psychological dependence. Physical signs of withdrawal such as headaches, irritation, nervousness, anxiety, and dizziness may occur upon abrupt discontinuation. It is most frequently measured in blood plasma, urine and commercial preparations using reversed phase HPLC, GC/FID and GC/MS.