HPLC Columns

HPLC columns are metal tubes generally 30 to 300mm in length with internal diameters of 2-5mm coated with a stationary phase on the inside. They are used to transport the analyte and the mobile phase and provide the environment in which separation is achieved. For example, in reversed phase HPLC, where the mobile phase is generally a mixture of polar solvents like acetonitrile and water, the stationary phase is often a silica-based packing covalently bonded with hydrophobic alkyl chains of C8 (octyl group) or C18 (octadecyl group). The reversed phase columns separate analytes on the basis of their hydrophobicity, with the more hydrophobic compounds being retained longer on the column. There are many variations on this theme including normal phase, affinity, ion exchange and size exclusion column stationary phases.

Contents

  • Alltech Adsorbosphere Columns
    Alltech Adsorbosphere┬« Columns are designed using high temperature bonding for exceptional surface coverage and stability for common applications. They are available in pore sizes from 60-90├ů and come in four varieties.
  • Alltech Alltima Normal Phase Columns
    Alltech Alltima Normal Phase columns are a line of HPLC columns in the Alltech HP family. The Alltech Alltima family are high quality general purpose columns. They are made with spherical silica, polymerically bonded and double endcapped for long lifeti
  • Alltech Alltima Reversed-Phase Columns
    Alltech Alltima Reversed Phase columns are a line of HPLC columns in the Alltech HP family. The Alltech Alltima family are high quality general purpose columns. They are made with spherical silica, polymerically bonded and double endcapped for long life
  • Alltech Apollo Columns
    Alltech Apollo columns are a line of HPLC columns in the Alltech HP family. The Attech Apollo family are batch-tested lower cost columns for routine analyses. They are made with spherical silica and endcapped with 100┼ pore sizes. They show broad pH st
  • Alltech Econosphere Columns
    Alltech Econosphere Columns are low-cost columns for common applications. They are available in 80┼ pore size and are acid-washed for low metal content. They are available with eleven packing phases.
  • Alltech HP HILIC Columns
    Alltech HP Hydrophobic Interaction HILIC columns are a line of HPLC columns in the Alltech HP family. These columns are made with high purity spherical silica to eliminate bleed and tailing. They are available with an HILIC packing phase in four differe
  • Alltech HP Normal Phase Columns
    Alltech HP Normal Phase columns are a line of HPLC columns in the Alltech HP family. These columns are made with high purity spherical silica to eliminate bleed and tailing. They are available with 4 different cyano and silica packing phases in five dif
  • Alltech HP Reversed-Phase Columns
    Alltech HP Reversed Phase columns are a line of HPLC columns in the Alltech HP family. These columns are made with high purity spherical silica to eliminate bleed and tailing. They are available with 12 different C18 and C8 packing phases in five differ
  • Alltech HPLC Column Families and Formats
    Alltech manufactures and supplies nine different families of HPLC columns and offers them in up to six different formats. The families and formats are briefly described here.
  • Alltech Macrosphere Columns
    The Alltech Macrosphere Columns are designed to separate proteins, peptides and polar synthetic compounds by size-exclusion. They are silica based (60-300┼).
  • Alltech Mixed-Mode Columns
    The Alltech Mixed-Mode Columns are designed to separate hydrophobic and ionic species on a single column. They are silica based (100┼).
  • Alltech Platinum Columns
    The Alltech Platinum Columns are designed to separate mixtures that a strongly hydrophobic phase cannot. They are silica based (100┼) and are available in Standard Platinum for neutral and moderately polar compounds and Platinum EPS (Extended Polar Selec
  • Alltech Prevail Carbohydrate ES Columns
    Alltech Prevail Carbohydrate ES columns are a line of polymer based reversed phase HPLC columns in the Alltech Prevail family for use in determining carbohydrates including mono- and oligosaccharides and sugar alcohols. They are available with a single p
  • Alltech Prevail Normal Phase Columns
    Alltech Prevail Normal Phase columns are a line of HPLC columns in the Alltech Prevail family for use with 100% organic to 100% aqueous mobile phases, allowing separation of highly polar analytes in aqueous mobile phases and hydrophobic analytes in organi
  • Alltech Prevail Organic Acid Columns
    Alltech Prevail Organic Acid columns are a line of silica based reversed phase HPLC columns in the Alltech Prevail family for use in determining organic acids. They are more efficient and operate at lower back-pressures than polymeric ion exchangers used
  • Alltech Prevail Reversed Phase Columns
    Alltech Prevail Reversed Phase columns are a line of HPLC columns in the Alltech Prevail family for use with 100% organic to 100% aqueous mobile phases, allowing separation of highly polar analytes in aqueous mobile phases and hydrophobic analytes in orga
  • Alltech ProSphere Affinity Columns
    The Alltech ProSphere family are a line of specialty packings for life science and proteomic applications. They are silica based (100┼ and 300┼) or polymer based. The Affinity columns use a polymer based packing phase to separate and purify proteins, pe
  • Alltech ProSphere Hydrophobic Interaction Columns
    The Alltech ProSphere family are a line of specialty packings for life science and proteomic applications. They are silica based (100┼ and 300┼) or polymer based. The Hydrophobic Interaction columns use a polymer based packing phase to separate proteins
  • Alltech ProSphere Ion Exchange Columns
    The Alltech ProSphere family are a line of specialty packings for life science and proteomic applications. They are silica based (100┼ and 300┼) or polymer based. The Ion Exchange columns use a polymer based packing phase to separate proteins, peptides,
  • Alltech ProSphere Ion Size Exclusion Columns
    The Alltech ProSphere family are a line of specialty packings for life science and proteomic applications. They are silica based (100┼ and 300┼) or polymer based. The Size Exclusion columns use a silica based packing phase to separate polysaccharides, p
  • Alltech ProSphere Reversed Phase Columns
    The Alltech ProSphere family are a line of specialty packings for life science and proteomic applications. They are silica based (100┼ and 300┼) or polymer based. The Reversed Phase columns use a silica based packing phase to separate proteins, peptides