HPLC/UV

The detector for an HPLC is the component that emits a response due to the eluting sample compound and subsequently signals a peak on the chromatogram. It is located immediately after to the stationary phase to detect compounds as they elute from the column. Ultraviolet (UV) detectors measure the ability of chromophores in compounds in a sample to absorb UV light. This can be accomplished at one or several wavelengths over the range from 190-400nm. Fixed wavelength detectors measure at a single wavelength, usually 254nm, variable wavelength detectors measure individual wavelengths sequentially, but can detect many over a wide range and diode arrays measure a spectrum of wavelengths simultaneously. The majority of organic compounds can be analyzed by UV detectors and most HPLC analyses are performed using UV detectors.

Contents


  • (4,5)-diphenyl-2-oxazolidinone by HPLC/UV
    (4,5)-diphenyl-2-oxazolidinone, Astec Application Report 4030 (4,5)-diphenyl-2-oxazolidinone, CAS No. 86286-50-2, a chiral compound used in asymmetric synthesis was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 column (250 x 4.6mm, Part No. 20024) using acetonitrile, water (30:70), 0.1%TEAA, pH 4.1. The enantiomers were detected by UV at 254nm.
  • 1,3-dicarboxylic acid allene by HPLC/UV
    1,3-dicarboxylic acid allene, Astec Application Report 271 1,3-dicarboxylic acid allene, CAS No. NR, a chiral compound used in asymmetric synthesis was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using 10% water in methanol, acetic acid, TEA (100:0.5:0.5). The enantiomers were detected by UV at 254nm.
  • 2,3-dimethyl tyrosine by HPLC/UV
    2,3-dimethyl tyrosine, Astec Application Report 208 2,3-dimethyl tyrosine, CAS No. NR, an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol, water (30:70). The enantiomers were detected by UV at 210nm.
  • 2,4-Dinitrophenylhydrazone Derivatives of Aldehydes and Ketones
    2,4-Dinitrophenylhydrazone Derivatives of Aldehydes and Ketones, Grom Application Number 04041 Twelve aldehydes and ketones in a mixture were derivatized with 2,4-DNPH and separated using a GROM-SIL 120 Butyl-1 ST HPLC column (150 x 4mm, Part No. GSBU10512S1504) by gradient elution with water/THF 90/10 in around 20 minutes and detected by UV at 360nm. The components separated were formaldehyde, acetaldehyde, acetone, acrolein, propionaldehyde, crotonaldehyde, methyl ethyl ketone, isobutyraldehyde, benzaldehyde, n-valeraldehyde, tolualdehyde and capronaldehyde.
  • 2,6-dimethyl tyrosine by HPLC/UV
    2,6-dimethyl tyrosine, Astec Application Report 209 2,6-dimethyl tyrosine, CAS No. NR, an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol, water (30:70). The enantiomers were detected by UV at 210nm.
  • 2-(2-chlorophenoxy) propionic acid by HPLC/UV
    2-(2-chlorophenoxy) propionic acid, Astec Application Report 5006 2-(2-chlorophenoxy) propionic acid, CAS No. NR, a herbicide was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol, acetic acid, TEA (100:0.2:0.1). The enantiomers were detected by UV at 230nm.
  • 2-(4-chlorophenoxy) propionic acid by Chirobiotic Tag Column-A
    2-(4-chlorophenoxy) propionic acid, Astec Application Report 5008 2-(4-chlorophenoxy) propionic acid, CAS No. NR, a herbicide was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol, acetic acid, TEA (100:0.2:0.1). The enantiomers were detected by UV at 230nm.
  • 2-(4-chlorophenoxy) propionic acid by Chirobiotic Tag Column-B
    2-(4-chlorophenoxy) propionic acid, Astec Application Report 5007 2-(4-chlorophenoxy) propionic acid, CAS No. NR, a herbicide was separated into its two respective enantiomers by isocratic elution in around 5 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using water, methanol, acetic acid, (85:15), 0.1% TEAA. The enantiomers were detected by UV at 220nm.
  • 2-bromo-3-methylbutyric acid by HPLC/UV
    2-bromo-3-methylbutyric acid, Astec Application Report 3000 2-bromo-3-methylbutyric acid, CAS No. 565-74-2, a chemical intermediate was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Chirobiotic R column (250 x 4.6mm, Part No. 13024) using ethanol, acetic acid, hexane and TEA (60:40:0.02:0.05). The enantiomers were detected by UV at 230nm.
  • 3a,4,5,6-Tetrahydrosuccinimido[3,4-b]acenaphthen-10-one -HPLC
    3a,4,5,6-Tetrahydrosuccinimido[3,4-b]acenaphthen-10-one, Astec Application Report 143 3a,4,5,6-Tetrahydrosuccinimido[3,4-b]acenaphthen-10-one, CAS No. 4756-92-7, a fungicide was separated into its two respective enantiomers by isocratic elution in around 21 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using ethanol and hexane (25:75). The enantiomers were detected by UV at 254nm.
  • 4-benzyl-2-oxazolidinone by HPLC/UV -Chirobiotic Tag Column
    4-benzyl-2-oxazolidinone, Astec Application Report 265 4-benzyl-2-oxazolidinone, CAS No. 90719-32-7, a chemical intermediate was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using 100% methanol. The enantiomers were detected by UV at 230nm.
  • 4-benzyl-2-oxazolidinone by HPLC/UV-Chirobiotic T Column
    4-benzyl-2-oxazolidinone, Astec Application Report 265 4-benzyl-2-oxazolidinone, CAS No. 90719-32-7, a chemical intermediate was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using water, methanol (20:80) 0.5% TEAA, pH 4.1. The enantiomers were detected by UV at 254nm.
  • 4-benzyl-2-oxazolidinone by HPLC/UV-Chirobiotic V Column
    4-benzyl-2-oxazolidinone, Astec Application Report 107 4-benzyl-2-oxazolidinone, CAS No. 90719-32-7, a chemical intermediate was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using hexane, ethanol (70:30) 0.5%. The enantiomers were detected by UV at 254nm.
  • 4-Hydroxymandelic acid by HPLC/UV Using Chirobiotic Tag Column
    4-Hydroxymandelic acid, Astec Application Report 5013 4-Hydroxymandelic acid, CAS No. NA a synthetic intermediate was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol, acetic acid and TEA (100:0.2:0.1). The enantiomers were detected by UV at 220nm.
  • 4-Methyl-5-phenyl-2-oxazolidinone by Using Chirobiotic Tag Colum
    4-Methyl-5-phenyl-2-oxazolidinone, Astec Application Report 5021 4-Methyl-5-phenyl-2-oxazolidinone, CAS No. NA a chiral auxiliary compound was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using 100% methanol. The enantiomers were detected by UV at 230nm.
  • 4-Phenyl-2-oxazolidinone by HPLC/UV Using Chirobiotic T Column
    4-Phenyl-2-oxazolidinone, Astec Application Report 261 4-Phenyl-2-oxazolidinone, CAS No. 90319-52-1 a synthetic intermediate was separated into its two respective enantiomers by isocratic elution in around 19 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using methanol and 0.5% TEAA in water, pH 4.1 (20:80). The enantiomers were detected by UV at 254nm.
  • 4-Phenyl-2-oxazolidinone by HPLC/UV Using Chirobiotic Tag Column
    4-Phenyl-2-oxazolidinone, Astec Application Report 5026 4-Phenyl-2-oxazolidinone, CAS No. 90319-52-1 a synthetic intermediate was separated into its two respective enantiomers by isocratic elution in around 16 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using 100% methanol. The enantiomers were detected by UV at 230nm.
  • 4-Phenyl-2-oxazolidinone by HPLC/UV Using Chirobiotic V Column
    4-Phenyl-2-oxazolidinone, Astec Application Report 150 4-Phenyl-2-oxazolidinone, CAS No. 90319-52-1 a synthetic intermediate was separated into its two respective enantiomers by isocratic elution in around 9 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 11024) using THF and 20mM ammonium nitrate in water, pH 5.5 (10:90). The enantiomers were detected by UV at 254nm.
  • 5,5-dimethyl-4-phenyl-2-oxazolidinone by HPLC/UV
    5,5-dimethyl-4-phenyl-2-oxazolidinone, Astec Application Report 260 5,5-dimethyl-4-phenyl-2-oxazolidinone, CAS No. 170918-42-0, a synthetic intermediate was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using methanol, water (20:80), 0.5% TEAA, pH 4.1. The enantiomers were detected by UV at 254nm.
  • 5-Methyl-5-phenylhydantoin by HPLC -Chirobiotic Tag Column
    5-Methyl-5-phenylhydantoin, Astec Application Report 5022 5-Methyl-5-phenylhydantoin, CAS No. 6843-49-8 a pharmaceutical intermediate compound was separated into its two respective enantiomers by isocratic elution in around 11 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using 100% methanol. The enantiomers were detected by UV at 220nm.
  • 5-Methyl-5-phenylhydantoin by HPLC -Chirobiotic V Column-A
    5-Methyl-5-phenylhydantoin, Astec Application Report 130 5-Methyl-5-phenylhydantoin, CAS No. 6843-49-8 a pharmaceutical intermediate compound was separated into its two respective enantiomers by isocratic elution in around 5 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using 100% ethanol. The enantiomers were detected by UV at 254nm.
  • 5-Methyl-5-phenylhydantoin by HPLC- Chirobiotic V Column-B
    5-Methyl-5-phenylhydantoin, Astec Application Report 131 5-Methyl-5-phenylhydantoin, CAS No. 6843-49-8 a pharmaceutical intermediate compound was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using THF and 20mM ammonium nitrate, pH 5.5 (10:90). The enantiomers were detected by UV at 254nm.
  • 7-aza-Tryptophan by HPLC/UV Using Chirobiotic T Column
    7-aza-Tryptophan, Astec Application Report 232 7-aza-Tryptophan, CAS No. NA a tryptophan analog was separated into its two respective enantiomers by isocratic elution in around 13 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and water (30:70). The enantiomers were detected by UV at 200nm.
  • a,a-Di-(2-naphthyl)-2-pyrrolidine methanol by HPLC/UV
    a,a-Di-(2-naphthyl)-2-pyrrolidine methanol, Astec Application Report 4007 a,a-Di-(2-naphthyl)-2-pyrrolidine methanol, CAS No. 130798-48-0, 127986-84-9, a catalyst ligand was separated into its two respective enantiomers by isocratic elution in around 30 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 SN column (250 x 4.6mm, Part No. 20524) using acetonitrile, methanol, acetic acid. TEA (98:2:0.03:0.02). The enantiomers were detected by UV at 254nm.
  • a-Methyl-a-phenyl succinimide by HPLC -Chirobiotic T Column
    a-Methyl-a-phenyl succinimide, Astec Application Report 249 a-Methyl-a-phenyl succinimide, CAS No. 1497-17-2 an anti-convulsant drug was separated into its two respective enantiomers by isocratic elution in around 5 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using hexane and ethanol (80:20). The enantiomers were detected by UV at 210nm.
  • a-Methyl-a-phenyl succinimide by HPLC -Chirobiotic Tag Column
    a-Methyl-a-phenyl succinimide, Astec Application Report 5020 a-Methyl-a-phenyl succinimide, CAS No. 1497-17-2 an anti-convulsant drug was separated into its two respective enantiomers by isocratic elution in around 18 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using ethanol and hexane (20:80). The enantiomers were detected by UV at 220nm.
  • a-Methylbenzylamine by HPLC -Cyclobond I 2000 DMP Column
    a-Methylbenzylamine, Astec Application Report 4014 a-Methylbenzylamine, CAS No. 98-94-0 a chiral intermediate compound was separated into its two respective enantiomers by isocratic elution in around 8 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 DMP column (250 x 4.6mm, Part No. 20724) using 100% ethanol. The enantiomers were detected by UV at 254nm.
  • a-Methylbenzylamine by HPLC/UV Using Chirobiotic V Column
    a-Methylbenzylamine, Astec Application Report 129 a-Methylbenzylamine, CAS No. 98-94-0 a chiral intermediate compound was separated into its two respective enantiomers by isocratic elution in around 16 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using hexane and IPA (90:10). The enantiomers were detected by UV at 254nm.
  • Alanine by HPLC/UV Using Chirobiotic T Column
    Alanine, Astec Application Report 200 Alanine, CAS No. 56-41-7, an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol, water (50:50). The enantiomers were detected by UV at 210nm.
  • Alanine by HPLC/UV Using Chirobiotic Tag Column
    Alanine, Astec Application Report 5001 Alanine, CAS No. 56-41-7, an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol, water (30:70). The enantiomers were detected by UV at 210nm.
  • Albuterol by HPLC/UV Using Chirobiotic T Column
    Albuterol, Astec Application Report 235 Albuterol, CAS No. 18559-94-9, a bronchodilator drug was separated into its two respective enantiomers by isocratic elution in around 15 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using acetonitrile, methanol, acetic acid, TEA (70:30:0.5:0.2). The enantiomers were detected by UV at 235nm.
  • Albuterol by HPLC/UV Using Chirobiotic V Column
    Albuterol, Astec Application Report 100 Albuterol, CAS No. 18559-94-9, a bronchodilator drug was separated into its two respective enantiomers by isocratic elution in around 15 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, TFA, ammonium hydroxide (100:0.01:0.01). The enantiomers were detected by UV at 230nm.
  • Alcohols, Aldehydes, Ketones
    Alcohols, Aldehydes, Ketones, Grom Application Number 01072 Alcohols, aldehydes and ketones in a standard mixture were separated using a GROM-SIL 100 ODS-0 AB HPLC column (125 x 4mm, Part No. GSOD00510S1204) by isocratic elution with water and acetonitrile 60/40 in around 17 minutes and detected by UV at 254nm. The compounds separated were benzyl alcohol, 2-phenoxyethanol, anisaldehyde, acetophenone, p-tolualdehyde, p-methylacetophenone, anisole and phenetole.
  • Alprenolol by HPLC/UV Using Chirobiotic T Column
    Alprenolol, Astec Application Report 264 Alprenolol, CAS No. 13655-52-2, an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 15 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024)) using methanol, acetic acid, TEA (100:0.1:0.1). The enantiomers were detected by UV at 254nm.
  • Alprenolol by HPLC/UV Using Chirobiotic V Column
    Alprenolol, Astec Application Report 101 Alprenolol, CAS No. 13655-52-2, an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, acetic acid, TEA (100:0.01:0.01). The enantiomers were detected by UV at 254nm.
  • Amino Acid Analysis by Precolumn Derivatization with DABS-Cl
    Separation of Amino Acids by HPLC after Precolumn Derivatisation with DABS-Cl Protein Hydrolysate Standard, Grom Application Number 10068a Sixteen amino acids in a protein hydrolysate standard were separated using a GROM-SIL DABS-1 column (200 x 4.6mm, Part No. GSDA10410S2004) after precolumn derivatization with DABS-Cl by gradient elution with 24mM sodium acetate, pH 6.6 and acetonitrile 80/20 and acetonitrile and 2-propanol 40/60 in around 26 minutes and detected by UV at 436nm. The amino acids separated were aspartic acid, glutamic acid, serine, threonine, glycine, alanine, arginine, proline, valine, methionine, isoleucine, leucine, phenylalanine, cysteine, lysine and histidine.
  • Amino Acid Analysis by Precolumn Derivatization with PITC
    Amino Acid Analysis by Precolumn Derivatization with PITC, Grom Application Number 10065 Eighteen amino acids in a standard mixture were separated using a GROM-SIL ODS-2 FE column (125 x 4mm, Part No. GSOD20308S1204) after precolumn derivatization with PITC by gradient elution with 50mM sodium acetate, pH 6.5 and 50mM sodium acetate, pH 6.5, acetonitrile 50/50 in around 9 minutes and detected by UV at 254nm. The amino acids separated were aspartic acid, glutamic acid, serine, glycine, threonine, alanine, proline, histidine, arginine, tyrosine, valine, methionine, cysteine, isoleucine, leucine, phenylalanine, tryptophan and lysine.
  • Amino Acids in Beer by Precolumn Derivatization with DABS-Cl
    Determination of Amino Acids in Beer by HPLC after Precolumn Derivatisation with DABS-Cl, Grom Application Number 10068d Amino acids in beer were separated using a GROM-SIL DABS-1 column (200 x 4.6mm, Part No. GSDA10410S2004) after precolumn derivatization with DABS-Cl by gradient elution with 24mM sodium acetate, pH 6.6 and acetonitrile 80/20 and acetonitrile and 2-propanol 40/60 in around 33 minutes and detected by UV at 436nm. The amino acids separated were not identified.
  • Amino Acids in Orange Juice by Precolumn Derivatization with DAB
    Determination of Amino Acids in Urine by HPLC after Precolumn Derivatisation with DABS-Cl, Grom Application Number 10068c Amino acids in an orange juice sample were separated using a GROM-SIL DABS-1 column (200 x 4.6mm, Part No. GSDA10410S2004) after precolumn derivatization with DABS-Cl by gradient elution with 24mM sodium acetate, pH 6.6 and acetonitrile 80/20 and acetonitrile and 2-propanol 40/60 in around 33 minutes and detected by UV at 436nm. The amino acids separated were not identified. The title of the application note appears to have been repeated from application note 10068b.
  • Amino Acids in Urine by Precolumn Derivatization with DABS-Cl
    Determination of Amino Acids in Urine by HPLC after Precolumn Derivatisation with DABS-Cl, Grom Application Number 10068b Amino acids in a urine sample were separated using a GROM-SIL DABS-1 column (200 x 4.6mm, Part No. GSDA10410S2004) after precolumn derivatization with DABS-Cl by gradient elution with 24mM sodium acetate, pH 6.6 and acetonitrile 80/20 and acetonitrile and 2-propanol 40/60 in around 33 minutes and detected by UV at 436nm. The amino acids separated were not identified.
  • Amphetamine by HPLC/UV
    Amphetamine, Astec Application Report 4000 Amphetamine, CAS No. 300-62-9, a CNS stimulant drug was separated into its two respective enantiomers after derivatization with AQC by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 DMP column (250 x 4.6mm, Part No. 20724) using water, acetonitrile, TEAA at pH 4. (45:55:0.01). The enantiomers were detected by UV at 230nm.
  • Analgesics, Antipyretics – HPLC Analysis of a Pill
    Analgesics, Antipyretics HPLC Analysis of a Pill, Grom Application Number 01032a Analgesic and antipyretic compounds in a pill (paracetamol, caffeine and acetylsalicylic acid) were separated using a GROM-SIL 100 ODS-2 FE HPLC column (125 x 4.6mm, Part No. GSOD20510S1205) by isocratic elution with acetonitrile and 0.1%TFA in water 15/85 in around 10 minutes and detected by UV at 274nm with acetylsalicylic acid detected at 227nm.
  • Analysis of 20 Pesticides by Reversed Phase HPLC
    Analysis of Pesticides by Reversed Phase HPLC, Grom Application Number 10070 A mixture of twenty pesticides was separated using a GROM-SIL PE-1 HPLC column (250 x 4mm, Part No. GSPE10512S2504) by gradient elution with 0.1mM ammonium acetate and acetonitrile in around 65 minutes and detected by UV at 230nm. The compounds separated were crimidine, bromacil, simacine, hexazinone, metabenzthiazurone, chlortolurone, atrazine, isoproturone, metobromurone, butorone, ametryn, propazine, linurone, terbutylazine, prometone, clorpropham, terbutryne, metholachlor, pentachlorophenol and mecoprop.
  • Analysis of Aldehydes and Ketones in an Auto Emissions Test Mix
    Analysis of Aldehydes and Ketones (all 14 Components of the Auto Emissions Mix Separated), Grom Application Number 10135 Fourteen aldehydes and ketones in an auto emissions test mixture were derivatized with 2,4-DNPH and separated using a GROM-SIL-AK HPLC column (250 x 4mm, Part No. GSAK10511S2504) by gradient elution with 100% water and with acetonitrile/THF 75/25 in around 30 minutes and detected by AUFS at 365nm. The components separated were formaldehyde, acetaldehyde, acetone, acrolein, propionaldehyde, crotonaldehyde, 2-butanone, methacrolein, butyraldehyde, benzaldehyde, cyclohexanone, valeraldehyde, p-tolualdehyde and hexaldehyde.
  • Analysis of Alkaloids by HPLC
    Alkaloids, Grom Application Number 01013 A mixture of nine alkaloid compounds was separated using a GROM-SIL 120 ODS-5 ST HPLC column (150 x 4mm, Part No. GSOD50512S1504) by gradient elution with 20mM potassium biphosphate and acetonitrile 83/17 and 20mM potassium biphosphate and acetonitrile 60/40 in around 80 minutes and detected by UV at 210nm. The compounds separated were hordenine sulfate, pilocarpine chloride, scopolamine bromide, atropine sulfate, quinine, sparteine sulfate, cinchonine, papaverine chloride and narcotine chloride.
  • Analysis of Anti inflammatory Drugs
    Analysis of Antiinflammatory Drugs, Grom Application Number 01091 A mixture of four anti inflammatory drugs was separated using a GROM-SIL 120 ODS-3 CP column (250 x 4mm, Part No. GSOD30512S2504) by isocratic elution with 5 mM SDS pH 2.2 (with phosphoric acid) and acetonitrile 50/50 in around 25 minutes and detected by UV at 230nm. The compounds separated were isopropylmethylphenol, lidocaine, diphenhydramine and hexanophenone.
  • Analysis of Aspartame in Soft Drinks
    Analysis of Aspartame in Soft Drinks, Grom Application Number 03039 A mixture of aspartame, caffeine and benzoic acid found in soft drinks was separated using a GROM-SIL 120 Phenyl-1 FE column (150 x 4mm, Part No. GSPH10512S1504) by isocratic elution with 50mM potassium phosphate, acetonitrile and methanol 84/3/13 in around 11 minutes and detected by UV at 214nm.
  • Analysis of Barbiturates by HPLC
    HPLC Analysis of Barbiturates, Grom Application Number 01102 A mixture of six barbiturate drugs was separated using a GROM-SIL 120 ODS-5 ST column (150 x 4mm, Part No. GSOD50512S1504) by isocratic elution with water, methanol 40/60 in around 15 minutes and detected by UV at 230nm. The compounds separated were barbital, Phenobarbital, butabarbital, hexobarbital, amobarbital and secobarbital.
  • Analysis of Five Alkaloids by HPLC
    Alkaloids, Grom Application Number 01104 A mixture of five alkaloid compounds was separated using a GROM-SIL 100 ODS-2 FE HPLC column (250 x 4mm, Part No. GSOD20510S2504) by isocratic elution with 0.05M potassium biphosphate pH 2.8, 0.04M octane-1-sulfonate and methanol 40/60 in around 10 minutes and detected by UV at 220nm. The compounds separated were caffeine, codeine, atropine sulfate, papaverine and eupaverin chloride.
  • Analysis of Nine Polyphenols by HPLC
    Analysis of Polyphenols by HPLC, Grom Application Number 01125 Nine polyphenols in a standard were separated using a GROM-SIL 120 ODS-5 ST column (250 x 4mm, Part No. GSOD50512S2504) by gradient elution with 0.5% phosphoric acid and methanol 10/90 and acetonitrile in around 33 minutes and detected by UV at 254nm. The polyphenols separated were gallic acid, protocatechinic acid, catechin, chlorogenic acid, epicatechin, rutin, fisetin, quercetin and campferol.
  • Analysis of Opium Alkaloids by HPLC
    HPLC Analysis of Opium Alkaloids, Grom Application Number 06126 A mixture of six opium alkaloid compounds was separated using a GROM-SIL 100 Cyan-2 PR HPLC column (250 x 4mm, Part No. GSCN20510S2504) by isocratic elution with 1% ammonium acetate pH 5.8, acetonitrile and dioxane 80/10/10 in around 10 minutes and detected by UV at 220nm. The compounds separated were morphine, codeine, cryptopine, thebaine, narcotine and papaverine.
  • Analysis of Organochlorine Pesticides by HPLC
    Analysis of Pesticides- Organochlorines, Grom Application Number 01077 A mixture of four chlorinated pesticides was separated using a GROM-SIL 100 ODS-2 FE HPLC HPLC column (125 x 4mm, Part No. GSOD20310S1204) by isocratic elution with water and methanol 20/80 in around 11 minutes and detected by UV at 220nm. The compounds separated were dieldrin, heptachlor, DDT and aldrin.
  • Analysis of Pesticides by Reversed Phase HPLC-II
    Analysis of Pesticides by Reversed Phase HPLC-II, Grom Application Number 10071 A mixture of 26 pesticides was separated using a GROM-SIL PE-2 column (250 x 4mm, Part No. GSPE20312S2504) by gradient elution with 1mM ammonium acetate and acetonitrile in around 60 minutes and detected by UV at 230nm. The pesticides separated were desisopropylatrazine, metamitrone, fenurone, desethylatrazine, crimidine, metoxurone, simazine, metribuzine, cyanizine, methabenzthiazurone, chlortolurone, atrazine, monolinurone, isoproturone, metobromurone, propham, metazachlor, sebutylazine, propazine, terbutylazine, linurone, chloroxurone, prometryne, clorpropham, terbutryne and metolachlor.
  • Analysis of Phenylurea Herbicides by HPLC
    Analysis of Phenylurea Herbicides, Grom Application Number 10134 A mixture of nine phenylurea herbicides was separated using a GROM-SIL PE-3 HPLC column (250 x 4mm, Part No. GSPE304029S2504) by gradient elution with 80 microliters phosphoric acid (85%) and water and acetonitrile in around 17 minutes and detected by UV at 220nm. The compounds separated were thifensulfuronmethyl, metsulfuron methyl, methabenzthiazuron, chlortoluron, rimsulfuron, amidosulfuron, isoproturon, diuron and linuron.
  • Analysis of Triazine Pesticides by HPLC
    Analysis of Triazine Pesticides, Grom Application Number 02117 A mixture of ten triazine pesticides was separated using a GROM-SIL 120 Octyl-3 BA HPLC column (150 x 4mm, Part No. GSOC30312S1504) by isocratic elution with 0.1M ammonium acetate pH 6.0 and acetonitrile 67/33 in around 22 minutes and detected by UV at 220nm. The compounds separated were simazine, atratone, simetryn, atrazine, secbumetone, prometone, ametryn, promazine, prometryn and terbutryn.
  • Analysis of UV Absorbers
    Analysis of UV Absorbers, Grom Application Number 01108 UV absorbing compounds were separated using a GROM-SIL 120 ODS-3 CP column (250 x 4mm, Part No. GSOD30512S2504) by isocratic elution with 3 mM trimethylstearylammonium chloride and acetonitrile 15/85 in around 18 minutes and detected by UV at 280nm. The compounds separated were isopropyl-p-methoxy cinnamate, 2-hydroxy-4-methoxy benzophenone, 2-phenylbenzimidazol-5-sulfonic acid, 2-hydroxy-4-methoxy benzophenone-5-sulfonic acid, isoamyl-p-methoxy cinnamate, anthracene, ethyl-2,4-diisopropyl cinnamate, 2-ethylhexyl-p-(diethylamino) benzoate, 2-ethylhexyl-p-methoxy cinnamate, 1,2-(di-p-methoxycinnamyl)-3-(2-ethylhexyl) glyceride and 3,3,5-trimethylcyclohexyl-2-hydroxybenzoate.
  • Analytical Separation of Protein Mixture by HPLC
    Analytical Separation of Protein Mixture, Grom Application Number 10184 A mixture of six proteins was separated using a Novarose SE-100/17 HPLC column (300 x 8mm, Part No. N101 290) by isocratic elution with 0.05 M sodium phosphate, pH 7.0, 0.15M sodium chloride in around 2 hours and detected by UV at 214nm. The compounds separated were thyroglobulin, aldolase, ovalbumin, carbonic anhydrase, ribonuclease and insulin.
  • Arginine by HPLC/UV
    Arginine, Astec Application Report 201 Arginine, CAS No. 74-79-3, an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol, 100mM sodium phosphate, at pH 4.6 (20:80). The enantiomers were detected by UV at 210nm.
  • Arotinolol by HPLC/UV Using Chirobiotic T Column
    Arotinolol, Astec Application Report 237 Arotinolol, CAS No. 68377-92-4, an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 15 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using acetonitrile, methanol, acetic acid, TEA (55:45: 03: 0.2). The enantiomers were detected by UV at 230nm.
  • Arotinolol by HPLC/UV Using Chirobiotic V Column
    Arotinolol, Astec Application Report 102 Arotinolol, CAS No. 68377-92-4, an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 15 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 12024) using methanol, acetic acid, TEA (100:0.01:0.01). The enantiomers were detected by UV at 230nm.
  • Arotinolol by HPLC/UV Using Cyclobond I 2000 Column
    Arotinolol, Astec Application Report 4001 Arotinolol, CAS No. 68377-92-4, an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 column (250 x 4.6mm, Part No. 20024) using acetonitrile, methanol, acetic acid, TEA (90:10:0.3:0.2). The enantiomers were detected by UV at 230nm.
  • Asparagine by HPLC/UV Using Chirobiotic T Column
    Asparagine, Astec Application Report 5002 Asparagine, CAS No. 70-47-3, an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol, water (50:50). The enantiomers were detected by UV at 210nm.
  • Asparagine by HPLC/UV Using Chirobiotic Tag Column
    Asparagine, Astec Application Report 5002 Asparagine, CAS No. 70-47-3, an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol, water (30:70). The enantiomers were detected by UV at 210nm.
  • Aspartic Acid by HPLC/UV
    Aspartic Acid, Astec Application Report 204 Aspartic Acid, CAS No. 56-84-8, an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol, methanol, water, at pH 4.1 with acetic acid (70:20:10). The enantiomers were detected by UV at 210nm.
  • Atenolol by HPLC/UV Using Chirobiotic T Column
    Atenolol, Astec Application Report 238 Atenolol, CAS No. 29122-68-7, an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 15 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using acetonitrile, methanol, acetic acid, TEA (55:45:0.3, 0.2). The enantiomers were detected by UV at 230nm.
  • Atenolol by HPLC/UV Using Chirobiotic V Column
    Atenolol, Astec Application Report 103 Atenolol, CAS No. 29122-68-7, an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 15 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 12024) using methanol, acetic acid, TEA (100:0.01:0.01). The enantiomers were detected by UV at 230nm.
  • Atrolactic Acid by HPLC/UV Using Chirobiotic R Column
    Atrolactic Acid, Astec Application Report 3001 Atrolactic acid, CAS No. 515-30-0, an organic intermediate used in pharmaceutical manufacturing was separated into its two respective enantiomers by isocratic elution in around 25 minutes by reversed phase HPLC on an Astec Chirobiotic R column (250 x 4.6mm, Part No. 13024) using methanol, acetic acid, TEA (100:0.02:0.01). The enantiomers were detected by UV at 254nm.
  • Atrolactic Acid by HPLC/UV Using Chirobiotic Tag Column
    Atrolactic Acid, Astec Application Report 5003 Atrolactic acid, CAS No. 515-30-0, an organic intermediate used in pharmaceutical manufacturing was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol, acetic acid, TEA (100:0.1:0.1). The enantiomers were detected by UV at 230nm.
  • Bendroflumethiazide by HPLC/UV Using Chirobiotic T Column
    Bendroflumethiazide, Astec Application Report 239 Bendroflumethiazide, CAS No. 73-48-3, an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol, water (30:70). The enantiomers were detected by UV at 254nm.
  • Bendroflumethiazide by HPLC/UV Using Chirobiotic V Column
    Bendroflumethiazide, Astec Application Report 104 Bendroflumethiazide, CAS No. 73-48-3, an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using water, THF (10:90), 20mM ammonium nitrate, pH 5.5. The enantiomers were detected by UV at 254nm.
  • Bendroflumethiazide by HPLC/UV-Cyclobond I 2000 SN Column
    Bendroflumethiazide, Astec Application Report 4002 Bendroflumethiazide, CAS No. 73-48-3, an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 SN column (250 x 4.6mm, Part No. 20524) using water, hexane (30:70) 1% TEAA, pH 4.5. The enantiomers were detected by UV at 254nm.
  • Benoxaprofen by HPLC/UV
    Benoxaprofen, Astec Application Report 105 Benoxaprofen, CAS No. 67434-14-4, an anti-inflammatory drug was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using water, THF (10:90), 0.1% TEAA, pH 7. The enantiomers were detected by UV at 254nm.
  • Benzoates Separated by HPLC
    Benzoates, Grom Application Number 10162 Five benzoates in a standard were separated using a GROM-SIL ODS 0 AB column (33 x 4mm, Part No. GSOD00210S0404) by isocratic elution with water and acetonitrile 30/70 in around 45 seconds and detected by UV at 254nm. The benzoates separated were methylbenzoate, ethylbenzoate, propylbenzoate, butylbenzoate and pentyl-benzoate.
  • Benzoates Separated by HPLC Using Novel Zirconia Phases
    Gradient Separation of Benzoates by Novel Zirconia Phases, Grom Application Number 10175 Five benzoates in a standard were separated using a GROM-SIL Zirconia RP C column (50 x 2mm, Part No. GZOD20330S0502) by gradient elution with water and acetonitrile in around 3 minutes and detected by UV at 254nm. The benzoates separated were methylbenzoate, ethylbenzoate, propylbenzoate, butylbenzoate and pentyl-benzoate.
  • Benzoates Separated by HPLC Using Zirconia Phases
    Benzoates, Zirconia Phases, Grom Application Number 10172 Five benzoates in a standard were separated using a GROM-SIL RP P column (33 x 4mm, Part No. GZOD10330S0502) by isocratic elution with water and acetonitrile 65/35 in around 8 minutes and detected by UV at 254nm. The benzoates separated were methylbenzoate, ethylbenzoate, propylbenzoate, butylbenzoate and pentyl-benzoate.
  • Benzoin Methyl Ether by HPLC/UV
    Benzoin methyl ether, Astec Application Report 106 Benzoin methyl ether, CAS No. 3524-62-7, a pharmaceutical intermediate was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using water, THF (10:90), 0.1% TEAA, pH 7.0. The enantiomers were detected by UV at 254nm.
  • Biological Amines, Catecholamines and Amino Acids by HPLC
    Biological Amines, Catecholamines - Amino Acids, Grom Application Number 01033 A mixture of twelve catecholamines and amino acids was separated using a using a GROM-SIL 120 ODS-4 HE HPLC column (250 x 4mm, Part No. GSOD40512S2504) by gradient elution with 100mM sodium phosphate, pH 3.0 and acetonitrile in around 25 minutes and detected by UV at 210nm. The compounds separated were noradrenaline, adrenaline, D,L-3,4-dihydroxymandelic acid, D,L-3,4-dihydroxyphenylalanine, dopamine, tyrosine, D,L-4-hydroxy-3-methoxymandelic acid, phenylalanine, 4-hydroxy-3-methoxyphenylglycol, 5-hydroxyindole-3-acetic acid, vanillic acid and homovanillic acid.
  • Biotin by HPLC/UV Using Chirobiotic T Column
    Biotin, Astec Application Report 240 Bitoin, CAS No. 58-85-5, a vitamin was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using water, methanol (40:60), 0.1% sodium phosphate, pH 4.1. The enantiomers were detected by UV at 210nm.
  • Biotin by HPLC/UV Using Chirobiotic V Column
    Biotin, Astec Application Report 108 Bitoin, CAS No. 58-85-5, a vitamin was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using water, methanol (20:80), 0.1% sodium phosphate, pH 4.1. The enantiomers were detected by UV at 210nm.
  • Bromacil by HPLC/UV Using Chirobiotic T Column
    Bromacil, Astec Application Report 241 Bromacil, CAS No. 314-40-9, a herbicide was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using water, methanol (30:70). The enantiomers were detected by UV at 210nm.
  • Bromacil by HPLC/UV Using Chirobiotic V Column
    Bromacil, Astec Application Report 109 Bromacil, CAS No. 314-40-9, a herbicide was separated into its two respective enantiomers by isocratic elution in around 15 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using water, THF (10:90), 20mM ammonium nitrate, pH 5.5. The enantiomers were detected by UV at 254nm.
  • Bupivacaine by HPLC/UV
    Bupivacaine, Astec Application Report 110 Bupivacaine, CAS No. 2180-92-9, an anesthetic drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using water, methanol (95:5), 1% TEAA, pH 4.1. The enantiomers were detected by UV at 265nm.
  • Butorphanol by HPLC/UV
    Butorphanol, Astec Application Report 270 Butorphanol, CAS No. 42408-82-2, an analgesic drug was separated into its two respective enantiomers by isocratic elution in around 15 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using methanol (100), 0.1% ammonium formate. The enantiomers were detected by UV at 254nm.
  • Carnitine by HPLC/UV
    Carnitine, Astec Application Report 5005 Carnitine, CAS No. 461-06-3, an amino acid was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using water, methanol, acetic acid, (85:15), 0.1% TEAA, pH 6.0. The enantiomers were detected by UV at 210nm.
  • Chloroquine by HPLC/UV
    Chloroquine, Astec Application Report 111 Chloroquine, CAS No. 54-05-7, an anti malarial drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using water, methanol, acetic acid, TEA (100:1:1). The enantiomers were detected by UV at 240nm.
  • Chlorpheniramine by HPLC/UV
    Chlorpheniramine, Astec Application Report 4003 Chlorpheniramine, CAS No. 132-22-9, an anti histaminic drug was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 column (250 x 4.6mm, Part No. 20024) using water, acetonitrile, (10:90), 1% TEAA, pH 4.1. The enantiomers were detected by UV at 254nm.
  • Chlorthalidone by Chirobiotic T Column
    Chlorthalidone, Astec Application Report 242 Chlorthalidone, CAS No. 77-36-1, a diuretic drug was separated into its two respective enantiomers by isocratic elution in around 35 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using water, methanol, (10:90), 0.1% TEAA, pH 4.1. The enantiomers were detected by UV at 254nm.
  • Chlorthalidone by Cyclobond I 2000 RSP Column
    Chlorthalidone, Astec Application Report 4004 Chlorthalidone, CAS No. 77-36-1, a diuretic drug was separated into its two respective enantiomers by isocratic elution in around 25 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 RSP column (250 x 4.6mm, Part No. 20324) using water, acetonitrile, (5:95), 1% TEAA, pH 4.1. The enantiomers were detected by UV at 254nm.
  • Ciprofibrate by HPLC/UV
    Ciprofibrate, Astec Application Report 4029 Ciprofibrate, CAS No. 52214-84-3, an antihyperlipoproteinemic drug was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 column (250 x 4.6mm, Part No. 20024) using acetonitrile, methanol, acetic acid, TEA (95:5:0.3:0.2). The enantiomers were detected by UV at 254nm.
  • Citalopram by HPLC/UV
    Citalopram, Astec Application Report 112 Citalopram, CAS No. 59729-33-8, an anti depressant drug was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, acetic acid, TEA (100:0.1.3:0.1). The enantiomers were detected by UV at 240nm.
  • Citrulline by HPLC/UV Using Chirobiotic T Column
    Citrulline, Astec Application Report 206 Citrulline, CAS No. 372-75-8, an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol, water (30:70). The enantiomers were detected by UV at 210nm.
  • Citrulline by HPLC/UV Using Chirobiotic Tag Column
    Citrulline, Astec Application Report 5009 Citrulline, CAS No. 372-75-8, an amino acid was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol, water (60:40). The enantiomers were detected by UV at 210nm.
  • Clenbuterol by HPLC/UV Using Chirobiotic V Column
    Clenbuterol, Astec Application Report 113 Clenbuterol, CAS No. 37148-27-9, a bronchodilator drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, acetic acid, TEA (100:0.01:0.01). The enantiomers were detected by UV at 254nm.
  • Clenbuterol by HPLC/UV Using Chirobiotic T Column
    Clenbuterol, Astec Application Report 263 Clenbuterol, CAS No. 37148-27-9, a bronchodilator drug was separated into its two respective enantiomers by isocratic elution in around 15 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using methanol, acetic acid, TEA (100:0.1:0.1). The enantiomers were detected by UV at 254nm.
  • Color Additives-Dyes
    Color Additives-Dyes, Grom Application Number 01001 Eleven dye compounds used as color additives were separated using a GROM-SIL 120 ODS-5 ST HPLC column (150 x 4mm, Part No. GSOD50512S1504) by gradient elution with 10mM ammonium phosphate, pH 6.0/methanol 90/10 and 10mM ammonium phosphate, pH 6.0/methanol 20/30 in around 40 minutes and detected by UV at 254nm. The compounds separated were tartrazine, amaranth, indigo carmine, new coccine, sunset yellow, fast green, brillian blue, erythosine, acid red, phloxine and rose bengale.
  • Coumachlor by HPLC/UV
    Coumachlor, Astec Application Report 114 Coumachlor, CAS No. 81-82-3, a rodenticide was separated into its two respective enantiomers by isocratic elution in around 25 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using THF, 20mM ammonium nitrate (25:75). The enantiomers were detected by UV at 254nm.
  • Coumafuryl by HPLC/UV
    Coumafuryl, Astec Application Report 115 Coumafuryl, CAS No. 117-52-2, a rodenticide was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using THF, 20mM ammonium nitrate, pH 5.5 (10:90). The enantiomers were detected by UV at 254nm.
  • Cresols + Phenol by HPLC/UV
    Cresols + Phenol, Astec Application Report 4006 Cresols + Phenol, CAS No. NR, three methylated phenols and phenol were separated into its respective enantiomers by isocratic elution in around 25 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 column (250 x 4.6mm, Part No. 20024) using methanol, water (10:90). The enantiomers were detected by UV at 254nm.
  • Cyclophosphamide by HPLC/UV
    Cyclophosphamide, Astec Application Report NEED Cyclophosphamide, CAS No. 50-18-0, an anti neoplastic drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol, THF, water (5:15:90). The enantiomers were detected by UV at 201nm.
  • Cysteine by HPLC/UV
    Cysteine, Astec Application Report 5010 Cysteine, CAS No. 52-90-4, an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol, water (60:40). The enantiomers were detected by UV at 230nm.
  • D,L-Proline by HPLC/UV Using Cyclobond II Column
    D,L-Proline, Astec Application Report 4021 D,L-Proline, CAS No. 606-36-9 an amino acid was separated after derivatization with FMOC into its two respective enantiomers by isocratic elution in around 17 minutes by reversed phase HPLC on an Astec Cyclobond II column (250 x 4.6mm, Part No. 41020) using acetonitrile, methanol, acetic acid and TEA (95:5:0.3:0.2). The enantiomers were detected by UV at 254nm.
  • Desalting of Alcohol Dehydrogenase
    Desalting of Alcohol Dehydrogenase, Grom Application Number 10186 Alcohol dehydrogenase was desalted using a Novarose SE-100/17 HPLC column (300 x 8mm, Part No. N101 290) by isocratic elution with 0.05M sodium phosphate (pH 6.7, 0.1M sodium chloride) in around 30 minutes and detected by UV at 214nm.
  • Determination of Bradykinins by Reversed Phase HPLC
    Determination of Bradykinins by Reversed Phase Chromatography, Grom Application Number 05119 A mixture of four bradykinins was separated using a GROM-SIL 300 TMS-2 CP column (250 x 4mm, Part No. GSTM20530S2504) by gradient elution with 0.1% TFA in water and 0.1% TFA in acetonitrile in around 8 minutes and detected by UV at 214nm. The bradykinins separated were lys-bradykinin, bradykinin, met-lys-bradykinin and des-arg-bradykinin.
  • Determination of Broncholytics-Xanthines by HPLC
    Analysis of Broncholytics-Xanthines by HPLC, Grom Application Number 01086 A mixture of five broncholytic xanthine compounds was separated using a GROM-SIL 120 ODS-4 HE column (250 x 4mm, Part No. GSOD40512S2504) by isocratic elution with 0.4% sodium acetate and acetonitrile 85/15 in around 10 minutes and detected by UV at 254nm. The broncholytic xanthine compounds separated were theobromine, theophylline, B-hydroxyethyltheophylline, 7-B-hydroxypropyltheophylline and caffeine.
  • Determination of Eight Nucleotides by HPLC
    Nucleotides, Grom Application Number 10180 A mixture of eight nucleotides was separated using a GROM-SIL 100 ODS-2 FE HPLC column (33 x 4.6mm, Part No. GSOD20210S0405) by gradient elution with 30mM potassium phosphate and potassium biphosphate/0.5M TBA pH 6.0 and 30mM potassium phosphate and potassium biphosphate/0.5M TBA pH 6.0 and acetonitrile 60/40 in around 7 minutes and detected by UV at 254nm. The compounds separated were UMP, GMP, AMP, NADP, ADP, CTP, GTP and ATP.
  • Determination of Food Additives by HPLC
    Determination of Food Additives by HPLC, Grom Application Number 01109 A mixture of eight food additive compounds was separated using a GROM-SIL 100 ODS-2 FE HPLC column (100 x 4mm, Part No. GSOD20310S1004) by isocratic elution with 0.05M sodium phosphate pH 4.5 and 2.5mM cetyl trimethylammonium chloride, methanol and acetonitrile 31/15/10 in around 20 minutes and detected by UV at 233nm. The compounds separated were DHA, ethyl PHBA, isopropyl PHBA, n-propyl PHBA, SOA, isobutyl PHBA, n-butyl PHBA and SA.
  • Determination of Nineteen Nucleotides by HPLC
    Nucleotides, Grom Application Number 01012 A mixture of nineteen nucleotides was separated using a GROM-SIL 120 ODS-5 ST HPLC column (250 x 4mm, Part No. GSOD50512S2504) by gradient elution with 0.2M TEAA, pH 6.6 and 0.2M TEAA, pH 6.6 and acetonitrile 95/5 in around 40 minutes and detected by UV at 260nm. The compounds separated were 50-CMP, 50-UMP, 50-dCMP, 50-CDP, 50-GMP, 50-UDP, 50-IMP, 50-CTP, 50-UTP, 50-GDP, 50-TMP, 50-AMP, 50-dGMP, 50-GTP, 50-TDP, 50-ADP, 50-TTP, 50-dAMP and 50-ATP.
  • Determination of Nucleosides and Bases by HPLC
    Nucleosides and Bases, Grom Application Number 01005 A mixture of eighteen nucleosides and bases was separated using a GROM-SIL 100 ODS-5 ST HPLC column (250 x 4mm, Part No GSOD50512S2504) by gradient elution with 20mM ammonium acetate pH 3.5 and 20mM ammonium acetate pH 3.5 and methanol 90/10 in around 30 minutes and detected by UV. The compounds separated were cytosine, cytidine, uracil, deoxycytidine, guanine, adenine, hypoxanthine, uridine, xanthine, thymine, inosine, guanosine, deoxyinosine, deoxyguanosine, xanthosine, thymidine, adenosine, deoxyadenosine.
  • Determination of Nucleosides by HPLC
    Nucleosides, Grom Application Number 01005 A mixture of six nucleosides was separated using a GROM-SIL 120 ODS-5 ST HPLC column (150 x 4mm, Part No. GSOD50512S1504) by isocratic elution with 10mM sodium acetate pH 5.0, acetonitrile and THF 955/30/5 v/v in around 23 minutes and detected by UV at 254nm. The compounds separated were 2,6,8-trihydroxypurine (uric acid), hypoxanthine, xanthine, inosine, guanosine and adenosine.
  • Determination of the Hop Bitter Acids in Beer by HPLC
    Determination of the Hop Bitter Acids in Beer by HPLC, Grom Application Number 10100 Eight hop bitter acids in beer were separated using a GROM Beer Bitter HPLC column (125 x 4mm, Part No. GSBI10712S1204) by gradient elution with methanol and 647 mL methanol, 349 mL water and 4 mL phosphoric acid in around 40 minutes and detected by UV at 270nm. The compounds separated were co-isohumulon, n-isohumulon, ad-isohumulon, xanthohumulon, co-humulon, n/ad humulon, co-lupulon and n/ad lupulon.
  • Determination of Tricyclic Antidepressants by HPLC
    Determination of Tricyclic Antidepressants, Grom Application Number 06111 A mixture of seven tricyclic antidepressant drugs was separated using a GROM-SIL 120 Cyan-3 CP HPLC column (150 x 4mm, Part No. GSCN30512S1504) by isocratic elution with 0.012M potassium dihydrogen phosphate pH 6.7, methanol and acetonitrile 17/43/40 in around 11 minutes and detected by UV at 215nm. The compounds separated were trimpramine, doxepine, amitriptyline, imipramine, notriptyline, desipramine and protriptyline.
  • Devinol by HPLC/UV
    Devinol, Astec Application Report 116 Devinol, CAS No.15299-99-7, a herbicide was separated into its two respective enantiomers by isocratic elution in around 15 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using THF, water (20:80), 0.1% TEAA. The enantiomers were detected by UV at 254nm.
  • Dopamine by HPLC/UV Using Chirobiotic T Column
    Dopamine, Astec Application Report 210 Dopamine, CAS No.63-84-3, an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol, water (30:70). The enantiomers were detected by UV at 210nm. (CONFIRM)
  • Dopamine by HPLC/UV Using Chirobiotic Tag Column
    Dopamine, Astec Application Report 5011 Dopamine, CAS No.63-84-3, an amino acid was separated into its two respective enantiomers by isocratic elution in around 25 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol, water (30:70). The enantiomers were detected by UV at 220nm.
  • Dothiepin by HPLC/UV
    Dothiepin, Astec Application Report 4008 Dothiepin, CAS No.113-53-1, an anti depressant drug was separated into its two respective enantiomers by isocratic elution by reversed phase HPLC on an Astec Cyclobond I 2000 AC column (250 x 4.6mm, Part No. 12024CONFIRM) using acetonitrile, water (15:85), 1% TEAA, pH 4.1. The enantiomers were detected by UV at 254nm.
  • Eight Benzodiazepin Sedatives by HPLC
    Determination of Benzodiazepins by HPLC, Grom Application Number 02113 Eight benzodiazepin sedatives in a standard were separated using a GROM-SIL 100 Octyl-2 AB column (150 x 4mm, Part No. GSOC20510S1504) by isocratic elution with 0.1M ammonium acetate, pH 6.0, methanol and acetonitrile 57/26.5/16.5 in around 13 minutes and detected by UV at 245nm. The benzodiazepins separated were demoxepam, nitrazepam, n-desmethylchlordiazepoxide, oxazepam, chlordiazepoxide, n-desmethyldiazepam, temazepam and diazepam.
  • Eight Water Soluble Vitamins Separated by Gradient HPLC
    Water-Soluble Vitamins, Grom Application Number 01004 Eight water-soluble vitamins in a standard were separated using a GROM-SIL 120 ODS-5 ST column (150 x 4.6mm, Part No. GSOD50512S1504) by gradient elution with 20mM potassium phosphate, pH 6.6 and 20mM potassium phosphate, pH 6.6 and acetonitrile 80/20 in around 22 minutes and detected by UV at 254nm. The vitamins separated were L-ascorbic acid (Vitamin C), nicotinic acid, pyridoxine hydrochloride (Vitamin B6), nicotinamide, thiamine hydrochloride (Vitamin B1), folic acid, cyanocobalamin (Vitamin B12) and riboflavin.
  • Enilconazole (Imazali) by HPLC/UV
    Enilconazole (Imazali), Astec Application Report 4031 Enilconazole (Imazali), CAS No.35554-44-0, an antifungal compound was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 column (250 x 4.6mm, Part No. 20024) using acetonitrile, 10mm ammonium acetate (10:90), pH 3.8. The enantiomers were detected by UV at 254nm.
  • Fenofibric Acid by HPLC/UV
    Fenofibric Acid, Astec Application Report 4009 Fenofibric Acid, CAS No.49562-28-9, an antihyperlipoproteinemic drug was separated into its two respective enantiomers by isocratic elution in around 30 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 column (250 x 4.6mm, Part No. 20024) using acetonitrile, 0.1% TEAA (80:20), pH 3.8. The enantiomers were detected by UV at 254nm.
  • Fenoprofen by HPLC/UV
    Fenoprofen, Astec Application Report 117 Fenoprofen, CAS No.31879-05-7, an anti inflammatory drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using THF, 0.1% TEAA (10:90), pH 7. The enantiomers were detected by UV at 254nm.
  • Fenoprofen methyl ester by HPLC/UV
    Fenoprofen methyl ester, Astec Application Report 147 Fenoprofen methyl ester, CAS No. NR, an experimental anti inflammatory drug was separated into its two respective enantiomers by isocratic elution in around 12 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using THF, sodium citrate 20mM (10:90), pH 6.3. The enantiomers were detected by UV at 254nm.
  • Fenuterol by HPLC/UV
    Fenuterol, Astec Application Report 118 Fenuterol, CAS No 13392-18-2 a bronchodilator drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using THF, ammonium nitrate 20mM (10:90), pH 5.5. The enantiomers were detected by UV at 254nm.
  • Fifteen Pestiscides Separated by HPLC
    Analysis of Pesticides, Grom Application Number 02118 Fifteen pesticides in a standard were separated using a GROM-SIL 100 Octyl-4 FE column (150 x 4mm, Part No. GSOC40310S1504) by gradient elution with water and acetonitrile in around 12 minutes and detected by UV at 210nm. The pesticides separated were methomyl, oxamyl, fenurone, monurone, carburone, propoxur, carbaryl, fluometurone, diurone, propham, sidurone, linurone, chlorpropham, barban and neburone.
  • Five Anticonvulsant Drugs by HPLC
    Analysis of Anticonvulsant Drugs, Grom Application Number 01093 A mixture of five anticonvulsant drugs was separated using a GROM-SIL 120 ODS-4 HE column (125 x 4mm, Part No. GSOD40312S1204) by isocratic elution with 5 mM sodium phosphate, pH 5.6 and acetonitrile 70/30 in around 9 minutes and detected by UV at 210nm. The compounds separated were primidone, phenobarbitone, butobarbitone, carbamazepine and phenytoin.
  • Five Bile Acids by HPLC
    HPLC of Bile Acids, Grom Application Number 03115 Five bile acids in a standard were separated using a GROM-SIL 120 Phenyl-2 CP column (250 x 4mm, Part No. GSPH20512S2504) by isocratic elution with 0.05M sodium phosphate, pH 7.0, methanol and ethanol 40/50/10 in around 11 minutes and detected by UV at 200nm. The bile acids separated were ursodeoxycholic acid, cholic acid, chenodeoxycholic acid, deoxycholic acid and lithocholic acid.
  • Flavanone by HPLC/UV
    Flavanone, Astec Application Report 4010 Flavanone, CAS No 487-26-3 a flavanoid compound was separated into its two respective enantiomers by isocratic elution in around 12 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 DMP column (250 x 4.6mm, Part No. 20724) using hexane, ethanol (80:20). The enantiomers were detected by UV at 254nm.
  • Fluoxetine (Prozac) by HPLC/UV Using Chirobiotic V Column-A
    Fluoxetine (Prozac), Astec Application Report 156 Fluoxetine (Prozac), CAS No 54910-89-3 an antidepressant drug was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, acetic acid, TEA (100:0.02:0.01). The enantiomers were detected by UV at 254nm.
  • Fluoxetine (Prozac) by HPLC/UV Using Chirobiotic V Column-B
    Fluoxetine (Prozac), Astec Application Report 119 Fluoxetine (Prozac), CAS No 54910-89-3 an antidepressant drug was separated into its two respective enantiomers by isocratic elution by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, ammonium nitrate 20mM (90:10), pH 4. The enantiomers were detected by UV at 265nm.
  • Flurbiprofen by HPLC/UV
    Flurbiprofen, Astec Application Report 120 Flurbiprofen, CAS No 5104-49-4 an anti inflammatory drug was separated into its two respective enantiomers by isocratic elution in around 15 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using THF, ammonium nitrate 20mM (20:80), pH 5.5. The enantiomers were detected by UV at 254nm.
  • Folinic acid by HPLC/UV Using Chirobiotic T Column-A
    Folinic acid, Astec Application Report 245 Folinic acid, CAS No 58-05-9 a vitamin was separated into its two respective enantiomers by isocratic elution in around 15 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using methanol, acetic acid, TEA (100:1.0:0.5). The enantiomers were detected by UV at 230nm.
  • Folinic acid by HPLC/UV Using Chirobiotic T Column-B
    Folinic acid, Astec Application Report 246 Folinic acid, CAS No 58-05-9 a vitamin was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol, TEA 1% (50:50), pH 4.5. The enantiomers were detected by UV at 254nm.
  • Folinic acid by HPLC/UV Using Chirobiotic V Column
    Folinic acid, Astec Application Report 121 Folinic acid, CAS No 58-05-9 a vitamin was separated into its two respective enantiomers by isocratic elution in around 12 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, acetic acid, TEA (100:0.01:01). The enantiomers were detected by UV at 254nm.
  • Four Catecholamines by HPLC
    Catecholamines, Grom Application Number 01026 A mixture of four catecholamines was separated using a GROM-SIL 120 ODS-4 HE HPLC column (250 x 4mm, Part No. GSOD40512S2504) by isocratic elution with 0.1M sodium phosphate, pH 5.7 and acetonitrile 97/3 in around 16 minutes and detected by UV at 210nm. The compounds separated were noradrenaline, adrenaline, 3,4-dihydroxyphenylalanine (DOPA) and dopamine.
  • Four Water Soluble Vitamins Separated by HPLC
    Water-Soluble Vitamins, Grom Application Number 01010 Four water-soluble vitamins and related compounds in a standard were separated using a GROM-SIL 120 ODS-5 ST column (150 x 4.6mm, Part No. GSOD50512S1504) by isocratic elution with 50mM ammonium phosphate and acetonitrile 90/10 in around 17 minutes and detected by UV at 210nm. The vitamins and related compounds separated were p-aminobenzoic acid, biotin (Vitamin H), cyanocobalamin (Vitamin B12) and riboflavin (Vitamin B2).
  • Fullerenes by HPLC
    Fullerenes, Grom Application Number 03038 A mixture of two fullerene compounds were separated using a GROM-SIL 120 Phenyl-1 FE column (250 x 4mm, Part No. GSPH10512S1504) by isocratic elution with n-hexane and 2-propanol 50/50 in around 8 minutes and detected by UV at 350nm. The compounds separated were buckminsterfullerene (C60) and (5,6)-fullerene (C70).
  • Glutamic acid by HPLC/UV Using Chirobiotic T Column
    Glutamic acid, Astec Application Report 212 Glutamic acid, CAS No 56-86-03 an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol, TEAA 1% in water (80:20), pH 4.0. The enantiomers were detected by UV at 210nm.
  • Glutamine by HPLC/UV Using Chirobiotic T Column
    Glutamine, Astec Application Report 5012 Glutamine, CAS No 56-85-9 an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and water (50:50). The enantiomers were detected by UV at 210nm.
  • Glutamine by HPLC/UV Using Chirobiotic Tag Column
    Glutamine, Astec Application Report 5012 Glutamine, CAS No 56-85-9 an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol and water (60:40). The enantiomers were detected by UV at 210nm.
  • Herbicide Residues by HPLC
    Herbicides Residue Analysis by HPLC, Grom Application Number 01025 A mixture of herbicide residues, herbicide and related ocmpounds was separated using a GROM-SIL 100 ODS-4 HE column (250 x 4mm, Part No. GSO40512S2504) by isocratic elution with 22.5 mM potassium phosphate, pH 2.9 and acetonitrile 75/25 in around 15 minutes and detected by UV at 280nm. The compounds separated were 3,6-dichloro-2-MGTH-oxybenzoic acid, p-bromophenol, 2,4-dichlorophenoxyacetic acid and (+)-2-(4-chloro-2-MGTH-yl phenoxy) propionic acid.
  • High Speed Analysis of Sedatives by HPLC
    High Speed HPLC Analysis of Seditatives-Benzodiazepins, Grom Application Number 10130b Two benzodiazepin sedatives in a standard were separated using a GROM-SIL 100 ODS-2 FE column (33 x 4.6mm, Part No. GSOD20210S0405) by isocratic elution with 0.1% TFA in water and acetonitrile 65/35 in around 1.3 minutes and detected by UV at 254nm. The benzodiazepins separated were clonazepam and diazepam along with the preservative benzyl alcohol.
  • High Speed Analysis of Three Drugs by HPLC
    High Speed Analysis of Drugs, Grom Application Number 10183 A mixture of three drugs was separated using a GROM-Ruby C18 column (50 x 1mm, Part No. GSODR0211S0501) by isocratic elution with 20mM sodium phosphate, pH 2.5 and acetonitrile 90/10 in around 20 minutes and detected by UV at 254nm. The drugs separated were hypoxanthine, theobromine and theophylline.
  • High Speed HPLC Analysis of the Analgesic/Antipyretic Thomapyrin
    High Speed HPLC Analysis of the Analgesic/Antipyretic Thomapyrin Pill, Grom Application Number 10032b Analgesic and antipyretic compounds in a Thomapyrin pill (paracetamol, caffeine and acetylsalicylic acid) were separated using a GROM-SIL 100 ODS-2 FE HPLC column (33 x 4.6mm, Part No. GSOD20210S0405) by isocratic elution with 0.1%TFA in water and acetonitrile 85/15 in around 3 minutes and detected by UV at 274nm with acetylsalicylic acid detected at 227nm.
  • High Speed Separation of Three Nonsteroidal Anti-inflammatory Dr
    High Speed Analysis of Nonsteroid Anti-inflammatory Drugs, Grom Application Number 01031b Three nonsteroidal anti-inflammatory drugs were separated using a GROM-SIL 100 ODS-2 FE column (250 x 4mm, Part No. GSOD20210S0405) by isocratic elution with 0.1% TFA in water and acetonitrile 60/40 in around 3.8 minutes and detected by UV at 254nm. The compounds separated were ketoprofen, diclofenac and ibuprofen.
  • High Speed Separation of Twelve Phenols by HPLC
    High Speed HPLC Analysis of Phenols (EPA), Grom Application Number 10079 A mixture of twelve phenols was separated using a GROM-SIL 100 Norm PH-1 ST column (33 x 4.6mm, Part No. GSOD20210S0405) by gradient elution with 0.1% TFA in water and acetonitrile in around 4 minutes and detected by UV at 274nm. The compounds separated were phenol, 4-nitrophenol, 2-chlorophenol, 2,4-dinitrophenol, 2-nitrophenol, 2,4-dimethylphenol, 4-chloro-3-methylphenol, 2,4-dichlorophenol, 2-methyl-4,6-dinitrophenol, 2,3,5-trichlorophenol, 2,4,6-trichlorophenol and pentachlorophenol.
  • Histidine by HPLC/UV Using Chirobiotic T Column
    Histidine, Astec Application Report 213 Histidine, CAS No. 71-00-1 an amino acid was separated into its two respective enantiomers by isocratic elution in around 15 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and 160mM sodium phosphate, pH 4.5 (60:40). The enantiomers were detected by UV at 210nm.
  • Homophenylalanine by HPLC/UV Using Chirobiotic T Column
    Homophenylalanine, Astec Application Report 214 Homophenylalanine, CAS No. 1012-05-1 an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and water (10:90). The enantiomers were detected by UV at 210nm.
  • HPLC Analysis of Antiarrythmics
    HPLC Analysis of Antiarrythmics, Grom Application Number 01116 A mixture of four antiarrythmic drugs was separated using a GROM-SIL 120 ODS-3 CP column (125 x 4mm, Part No. GSOD30512S1204) by isocratic elution with 0.05% triethylammonium acetate pH 4.0 and acetonitrile 80/20 in around 13 minutes and detected by UV at 220nm. The compounds separated were procaine, lidocaine, quinidine sulfate and benzocaine.
  • HPLC Analysis of Antisense Oligonucleotides
    Antisense Oligonucleotides, Grom Application Number 10181 A mixture of antisense oligonucleotides was separated using a GROM-SIL 100 ODS-2 FE column (33 x 4.6mm, Part No. GSOD20210S0405) by gradient elution with 0.05 M TEAA, pH 7.0 and 0.05M TEAA, pH 7.0 and acetontrile 30/70 in around 6 minutes and detected by UV at 260nm.
  • HPLC Analysis of Cough and Cold Preparations
    HPLC Analysis of Cough and Cold Preparations, Grom Application Number 01088 Potassium gulacol sulfonate, acetominophen, caffeine, salicylamide, maleic acid chlorophenylamine, phenol, aspirin and ethenzamidein a cold and cough preparation were separated using a GROM-SIL, 120 ODS-3 CP HPLC column (250 x 4mm, Part No. GSOD30512S2504) by isocratic elution with 0.05M sodium phosphate/acetonitrile 20/80 at pH 2 in around 16 minutes and detected by UV at 280nm.
  • HPLC Analysis of Four Antihistamines
    Analysis of Antihistamines, Grom Application Number 01120 A mixture of four antihistamines was separated using a GROM-SIL 120 ODS-4 HE column (125 x 4mm, Part No. GSOD40512S1504) by isocratic elution with 0.1% triethylammonium acetate pH 4.0 and acetonitrile 40/60 in around 5 minutes and detected by UV at 220nm. The compounds separated were phenylpropanolamine, diphenylhydramine, pseudoephridine and promethazine.
  • HPLC Analysis of Glycosides in Crude Drugs
    Crude Drugs - Glycosides, Grom Application Number 01007 A mixture of eight glycosides found in crude drugs was separated using a GROM-SIL 120 ODS-5 ST column (250 x 4mm, Part No. GSOD50512S2504) by gradient elution with 20mM ammonium dihydrophosphate and methanol 95/5 and 20 mM ammonium dihydrophosphate and methanol 20/80 in around 40 minutes and detected by UV at 250nm. The glycosides separated were arbutin, salicin, esculine, strophantin G (ouabain), baicalein, barboloin, glycyrrhizin and digitoxin.
  • HPLC Analysis of Two Antibiotics
    Antibiotics, Grom Application Number 01035 A mixture of two antibiotic drugs was separated using a GROM-SIL 120 ODS-3 CP column (125 x 4mm, Part No. GSOD30510S1204) by isocratic elution with 10 mM oxalic acid and acetonitrile 75/25 in around 13 minutes and detected by UV at 340nm. The compounds separated were aureomycine and doxycycline.
  • HPLC of Quinones
    HPLC of Quinones, Grom Application Number 01097 A mixture of five quinone compounds was separated using a GROM-SIL 120 ODS-HE column (125 x 4mm, Part No. GSOD40312S1204) by isocratic elution with 20mM potassium dihydrogen phosphate pH 2.0 and acetonitrile 50/50 in around 6 minutes and detected by UV at 254nm. The compounds separated were p-benzoquinone, 2,5-dimethylbenzoquinone, 1,4-naphthoquinone, duroquinone and anthraquinone.
  • HPLC Separation of Angiotensins-Peptide Hormones
    Antibiotics, Grom Application Number 01011 A mixture of five peptide hormones was separated using a GROM-SIL 120 ODS-5 ST column (150 x 4mm, Part No. GSOD50512S1504) by isocratic elution with 20mM potassium dihydrogen phosphate and acetonitrile 80/20 in around 26 minutes and detected by UV at 220nm. The compounds separated were angiotensin III-RVYIHPF, angiotensin II-DRVYIHPF, [asn1, val15]-angiotensin I-NRVYVHPFHL, [val15]-angiotensin I-DRVYVHPFHL and angiotensin I-DRVYIHPFHL.
  • HPLC Separation of Eight Peptides and Proteins
    Separation of Peptides and Proteins, Grom Application Number 02051 A mixture of eight peptides and proteins was separated using a GROM-SIL 120 Octyl-6 MB column (250 x 4mm, Part No. GSOC60512S2504) by gradient elution with 0.1% TFA and acetonitrile in around 40 minutes and detected by UV at 200nm. The compounds separated were bradykinin, met-enkephalin, angiotensin I, leu-enkephalin, substance P, insulin, lysozyme and myoglobin.
  • HPLC Separation of Four Antibiotics
    HPLC Separation of Antibiotics, Grom Application Number 01107 A mixture of four antibiotic drugs was separated using a GROM-SIL 100 ODS-0 AB column (125 x 4mm, Part No. GSOD00510S1204) by isocratic elution with 0.1M sodium dihydrogen phosphate and acetonitrile 70/30. in around 13 minutes and detected by UV at 220nm. The compounds separated were amoxicillin, penicillin V, penicillin G and nafcillin.
  • HPLC Separation of Nine Antibiotics
    Antibiotics, Grom Application Number 01016 A mixture of nine antibiotic drugs was separated using a GROM-SIL 120 ODS-5 ST column (150 x 4mm, Part No. GSOD50512S1504) by gradient elution with 50mM ammonium phosphate and acetonitrile 90/10 and 50mM ammonium phosphate and acetonitrile 50/50 in around 33 minutes and detected by UV at 250nm. The compounds separated were ampicilline, cephalexine, oxytetracycline hydrochloride, tetracycline, chlorotetracycline, chloramphenichol, oxolinic acid, doxorubicine hydrochloride and nalidixic acid.
  • Hydrophobic Interaction Chromatography of Seven Proteins
    Hydrophobic Interaction Chromatography of Seven Proteins, Grom Application Number 09106 A mixture of seven proteins was separated using a GROM-SIL 300 HIC column (250 x 4.6mm, Part No. GSHI10730S2505) by gradient elution with 2.0M ammonium sulfate and 0.1M potassium phosphate, pH 6.8 and 0.lM potassium phosphate, pH 6.8 in around 31 minutes and detected by UV at 280nm. The proteins separated were cytochrome C, myoglobin, B-lactoglobulin, ribonuclease A, lysozyme, a-chymotrypsin and chymotrypsinogen.
  • Hydroxychloroquine by HPLC/UV Using Chirobiotic V Column
    Hydroxychloroquine, Astec Application Report 122 Hydroxychloroquine, CAS No. 118-42-3 an anti-malarial drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, acetic acid and TEA (100:1.0:1.0). The enantiomers were detected by UV at 254nm.
  • Ifosfamide by HPLC/UV Using Chirobiotic T Column
    Ifosfamide, Astec Application Report 247 Ifosfamide, CAS No. 3778-73-2 an anti-neoplastic drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using THF and water (10:90). The enantiomers were detected by UV at 205nm.
  • Indoprofen by HPLC/UV Using Chirobiotic V Column
    Indoprofen, Astec Application Report 148 Indoprofen, CAS No. 31842-01-0 an analgesic drug was separated into its two respective enantiomers by isocratic elution in around 13 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using THF and 20mM sodium citrate, pH 6.3 (10:90). The enantiomers were detected by UV at 254nm.
  • Ingredients in a Cough and Cold Medication
    Ingredients in a Cough and Cold Medication, Grom Application Number 01014 Acetominophen, caffeine, chloropheniramine malate, narcotine, ethenzamide in and brucetine in a cold and cough medication were separated using a GROM-SIL, 120 ODS-5 ST HPLC column (250 x 4mm, Part No. GSOD50512S2504) by isocratic elution with 0.1% phosphoric acid, methanol, acetonitrile 77/15/18 in around 16 minutes and detected by UV at 254nm.
  • Isocratic Separation of Polycyclic Aromatic Hydrocarbons (PAH’s)
    Isocratic separation of Polycyclic Aromatic Hydrocarbons (PAHs), Grom Application Number 10036 Sixteen polycyclic aromatic hydrocarbons in a mixture were separated using a GROM-SIL PAH-1 HPLC column (250 x 4mm, Part No. GSPA10530S2504) by isocratic elution with water and methanol 15/85 in around 16 minutes and detected by UV at 254nm. The compounds separated were naphthalene, acenaphthylene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, dibenzo(a,h)anthracene, benzo(g,h)perylene and indeno(1,2,3,c,d) pyrene.
  • Isocratical PTH-Amino Acid Analysis by Capillary HPLC
    Isocratical PTH-Amino Acid Analysis by Capillary HPLC, Grom Application Number 10066 Nineteen amino acids in a standard mixture were separated using a NovoGROM capillary column, Superspher C8 endcapped (250mm x 0.3mm, Part No. MSOC50406C2530) by isocratic elution with 21mM sodium acetate, pH 4, acetonitrile and 1,2-dichloromethane 68/31/0.5 v/v, elution time not reported and detected by UV at 254nm. The amino acids separated were aspartic acid, glutamic acid, asparagine, glutamine, serine, threonine, histidine, glycine, alanine, tyrosine, arginine, methionine, valine, proline, tryptophan, phenylalanine, isoleucine, lysine and leucine.
  • Isoleucine by HPLC/UV Using Chirobiotic Tag Column
    Isoleucine, Astec Application Report 5014 Isoleucine, CAS No. 73-32-5 an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol and water (30:70). The enantiomers were detected by UV at 210nm.
  • Isoleucine, Allo by HPLC/UV Using Chirobiotic Tag Column
    Isoleucine, allo, Astec Application Report 5015 Isoleucine, CAS No. 73-32-5 an amino acid was separated into its two respective allo enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol and water (85:15). The enantiomers were detected by UV at 210nm.
  • Isopromethazine by HPLC/UV Using Chirobiotic V Column
    Isopromethazine, Astec Application Report 124 Isopromethazine, CAS No. 303-14-0 an anti-histaminic drug was separated into its two respective enantiomers by isocratic elution in around 12 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, acetic acid and TEA (100:0.1:0.1). The enantiomers were detected by UV at 240nm.
  • Isoproterenol by HPLC/UV Using Chirobiotic T Column
    Isoproterenol, Astec Application Report 248 Isoproterenol, CAS No. 7683-59-2 a bronchodilator drug was separated into its two respective enantiomers by isocratic elution in around 12 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using acetonitrile, methanol, acetic acid and TEA (55:45:0.3:0.2). The enantiomers were detected by UV at 230nm.
  • Ketoprofen by HPLC/UV Using Chirobiotic V Column
    Ketoprofen, Astec Application Report 149 Ketoprofen, CAS No. 22071-15-4 an anti-inflammatory drug was separated into its two respective enantiomers by isocratic elution in around 11 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using THF and 20mM sodium citrate, pH 6.3 (10:90). The enantiomers were detected by UV at 254nm.
  • Ketorolac by HPLC/UV Using Chirobiotic T Column
    Ketorolac, Astec Application Report 272 Ketorolac, CAS No. 74103-06-3 an anti-inflammatory drug was separated into its two respective enantiomers by isocratic elution in around 5 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using methanol and ammonium acetate (100:0.1 w%). The enantiomers were detected by UV at 230nm.
  • Ketotifen by HPLC/UV Using Cyclobond I 2000
    Ketotifen, Astec Application Report 4033 Ketotifen, CAS No. 34580-13-7 an anti-asthmatic drug was separated into its two respective enantiomers by isocratic elution in around 7 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 column (250 x 4.6mm, Part No. 20024) using acetonitrile and 10mM ammonium acetate, pH 3.8 (10:90). The enantiomers were detected by UV at 275nm.
  • L-Proline by HPLC/UV Using Chirobiotic T Column
    Proline, Astec Application Report 224 Proline, CAS No. 147-85-3 an amino acid was separated into its two respective enantiomers by isocratic elution in around 4 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using 100% water. The enantiomers were detected by UV at 210nm.
  • Labetalol by HPLC/UV Using Chirobiotic V Column
    Labetalol, Astec Application Report 125 Labetalol, CAS No. 36894-69-6 an anti-hypertensive drug was separated into its four respective enantiomers by isocratic elution in around 27 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, acetic acid and TEA (100:0.15:0.05). The enantiomers were detected by UV at 240nm.
  • Leucine by HPLC/UV Using Chirobiotic T Column
    Leucine, Astec Application Report 215 Leucine, CAS No. 61-90-5 an amino acid was separated into its two respective enantiomers by isocratic elution in around 6 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and water (30:70). The enantiomers were detected by UV at 210nm.
  • Leucine by HPLC/UV Using Chirobiotic Tag Column
    Leucine, Astec Application Report 5016 Leucine, CAS No. 61-90-5 an amino acid was separated into its two respective enantiomers by isocratic elution in around 9 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol and water (30:70). The enantiomers were detected by UV at 210nm.
  • Limonene in Grapefruit Oil
    Limonene in Grapefruit Oil, Grom Application Number 01047 Limonene in grapefruit oil was separated using a GROM-SIL 80 ODS-2 FE column (250 x 4mm, Part No. GSOD20508S2504) by isocratic elution with water and methanol 50/50 in around 8 minutes and detected by UV at 254nm. No individual compounds were identified.
  • Lorazepam by HPLC/UV Using Chirobiotic T Column-A
    Lorazepam, Astec Application Report 273 Lorazepam, CAS No. 846-49-1 an anxiolytic drug was separated into its two respective enantiomers by isocratic elution in around 8 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using 100% methanol. The enantiomers were detected by UV at 254nm.
  • Lorazepam by HPLC/UV Using Chirobiotic T Column-B
    Lorazepam, Astec Application Report 268 Lorazepam, CAS No. 846-49-1 an anxiolytic drug was separated into its two respective enantiomers by isocratic elution in around 8 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using methanol, acetic acid and TEA (100:0.1:0.1). The enantiomers were detected by UV at 254nm. On column racemization was prevented by lowering the column temperature to <10C.
  • Lorazepam by HPLC/UV Using Chirobiotic Tag Column
    Lorazepam, Astec Application Report 5017 Lorazepam, CAS No. 846-49-1 an anxiolytic drug was separated into its two respective enantiomers by isocratic elution in around 8 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using 100% methanol. The enantiomers were detected by UV at 220nm.
  • Luciferin by HPLC/UV Using Chirobiotic V Column
    Luciferin, Astec Application Report 126 Luciferin, CAS No. NA a biolumin was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using THF and 20mM ammonium nitrate, pH 5.5 (10:90). The enantiomers were detected by UV at 254nm.
  • Lysine by HPLC/UV Using Chirobiotic T Column
    Lysine, Astec Application Report 217 Luciferin, CAS No. 56-87-1 an amino acid was separated into its two respective enantiomers by isocratic elution in around 7 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and 100mM sodium phosphate, pH 4.6 (20:80). The enantiomers were detected by UV at 210nm.
  • m-Tyrosine by HPLC/UV Using Chirobiotic T Column
    m-Tyrosine, Astec Application Report 228 m-Tyrosine, CAS No. 587-33-7 an amino acid was separated into its two respective enantiomers by isocratic elution in around 7 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and water (10:90). The enantiomers were detected by UV at 210nm.
  • m-Tyrosine by HPLC/UV Using Chirobiotic Tag Column
    m-Tyrosine, Astec Application Report 5033 m-Tyrosine, CAS No. 587-33-7 an amino acid was separated into its two respective enantiomers by isocratic elution in around 40 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol and water (30:70). The enantiomers were detected by UV at 220nm.
  • Mandelic Acid by HPLC/UV Using Chirobiotic Tag Column
    Mandelic acid, Astec Application Report 5018 Mandelic acid, CAS No. 90-64-2 a chiral antiseptic compound intermediate was separated into its two respective enantiomers by isocratic elution in around 15 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol, acetic acid and TEA (100:0.1:0.1). The enantiomers were detected by UV at 230nm.
  • Mephenytoin by HPLC/UV Using Chirobiotic V Column
    Mephenytoin, Astec Application Report 152 Mephenytoin, CAS No. 50-12-4 an anti-convulsant drug was separated into its two respective enantiomers by isocratic elution in around 13 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using hexane and ethanol (95:5). The enantiomers were detected by UV at 230nm.
  • Mephobarbital by HPLC/UV Using Chirobiotic V Column
    Mephobarbital, Astec Application Report 127 Mephobarbital, CAS No. 115-38-8 an anti-convulsant drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using ethanol and hexane (25:75). The enantiomers were detected by UV at 254nm.
  • Methadone by HPLC/UV Using Cyclobond I 2000 RSP Column-A
    Methadone, Astec Application Report 4012 Methadone, CAS No. 76-99-3 an analgesic drug was separated into its two respective enantiomers by isocratic elution in around 17 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 SP column (250 x 4.6mm, Part No. 20324) using acetonitrile and 1% TEAA in water, pH 4.1 (15:85). The enantiomers were detected by UV at 254nm.
  • Methadone by HPLC/UV Using Cyclobond I 2000 RSP Column-B
    Methadone, Astec Application Report 4012 Methadone, CAS No. 76-99-3 an analgesic drug was separated into its two respective enantiomers by isocratic elution in around 12 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 RSP column (250 x 4.6mm, Part No. 20324) using acetonitrile and 10mM ammonium acetate in water, pH 3.6 (15:85). The enantiomers were detected by UV at 292nm.
  • Methadone by HPLC/UV Using Cyclobond I 2000 SP Column
    Methadone, Astec Application Report 4012 Methadone, CAS No. 76-99-3 an analgesic drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 SP column (250 x 4.6mm, Part No. 20224) using acetonitrile and 1% TEAA in water, pH 4.1 (15:85). The enantiomers were detected by UV at 254nm.
  • Methamphetamine by HPLC/UV Using Cyclobond I 2000 DMP Column
    Methamphetamine, Astec Application Report 4013 Methamphetamine, CAS No. 537-46-2 an anorexic drug was separated into its two respective enantiomers by isocratic elution in around 40 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 DMP column (250 x 4.6mm, Part No. 20724) using acetonitrile and 0.1% TEAA in water, pH 4.1 (35:65). The enantiomers were detected by UV at 254nm.
  • Methionine by HPLC/UV Using Chirobiotic T Column
    Methionine, Astec Application Report 219 Methionine, CAS No. 63-68-3 an amino acid was separated into its two respective enantiomers by isocratic elution in around 6 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and water (20:80). The enantiomers were detected by UV at 210nm.
  • Methionine by HPLC/UV Using Chirobiotic Tag Column
    Methionine, Astec Application Report 5019 Methionine, CAS No. 63-68-3 an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol and water (30:70). The enantiomers were detected by UV at 210nm.
  • Methyl-phenyl sulfoxide by HPLC/UV Using Chirobiotic Tag Column
    Methyl-phenyl sulfoxide, Astec Application Report 5023 Methyl-phenyl sulfoxide, CAS No. 1193-82-4 a compound used in amino acid studies was separated into its two respective enantiomers by isocratic elution in around 16 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using ethanol and hexane (40:60). The enantiomers were detected by UV at 260nm.
  • Methylphenidate (Ritalin) by HPLC/UV Using Chirobiotic V Column
    Methylphenidate (Ritalin), Astec Application Report 128 Methylphenidate (Ritalin), CAS No. 113-45-1 a CNS stimulant was separated into its two respective enantiomers by isocratic elution in around 6 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol and 1.0% TEAA in water (95:5). The enantiomers were detected by UV at 220nm.
  • Metoprolol by HPLC/UV Using Chirobiotic T Column-A
    Metoprolol, Astec Application Report 274 Metoprolol, CAS No. 37350-58-6 an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using methanol and 0.1% ammonium TFA (100:0.1 w%). The enantiomers were detected by UV at 230nm.
  • Metoprolol by HPLC/UV Using Chirobiotic T Column-B
    Metoprolol, Astec Application Report 274 Metoprolol, CAS No. 37350-58-6 an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 9 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using acetonitrile, methanol, acetic acid and TEA (55:45:0.3:0.2). The enantiomers were detected by UV at 230nm.
  • Metoprolol by HPLC/UV Using Cyclobond I 2000 Column-A
    Metoprolol, Astec Application Report 4035 Metoprolol, CAS No. 37350-58-6 an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 column (250 x 4.6mm, Part No. 20024) using acetonitrile, methanol, acetic acid and TEA (95:5:0.3:0.2). The enantiomers were detected by UV at 254nm.
  • Metoprolol by HPLC/UV Using Cyclobond I 2000 Column-B
    Metoprolol, Astec Application Report 4015 Metoprolol, CAS No. 37350-58-6 an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 column (250 x 4.6mm, Part No. 20024) using acetonitrile, methanol, acetic acid and TEA (90:10:0.3:0.2). The enantiomers were detected by UV at 230nm.
  • Mianserin by HPLC/UV Using Chirobiotic V Column
    Mianserin, Astec Application Report 154 Mianserin, CAS No. 24219-97-4 an antidepressant drug was separated into its two respective enantiomers by isocratic elution in around 17 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, acetic acid and TEA (100:0.1:0.1). The enantiomers were detected by UV at 254nm.
  • Miconazole by HPLC/UV Using Cyclobond I 2000 RSP Column
    Miconazole, Astec Application Report 4016 Miconazole, CAS No. 22916-47-8 an antifungal compound was separated into its two respective enantiomers by isocratic elution in around 15 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 column (250 x 4.6mm, Part No. 20324) using acetonitrile and 1% TEA in water, pH 4.0 (20:80). The enantiomers were detected by UV at 230nm.
  • Mosapride by HPLC/UV Using Chirobiotic V Column-A
    Mosapride, Astec Application Report 145 Mosapride, CAS No. 112885-41-3 a gastrointestinal regulator compound was separated into its two respective enantiomers by isocratic elution in around 13 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, acetic acid and TEA (100:0.03:0.01). The enantiomers were detected by UV at 254nm.
  • Mosapride by HPLC/UV Using Chirobiotic V Column-B
    Mosapride, Astec Application Report 146 Mosapride, CAS No. 112885-41-3 a gastrointestinal regulator compound was separated into its two respective enantiomers by isocratic elution in around 9 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using THF and 20mM ammonium nitrate, pH 5.5 (40:60). The enantiomers were detected by UV at 254nm.
  • N-1(1-Phenylethyl) maleimide by HPLC/UV -Chirobiotic R Column
    N-1(1-Phenylethyl) maleimide, Astec Application Report 3002 N-1(1-Phenylethyl) maleimide, CAS No. R-(+) 6129-15-3 and S-(-) 60925-76-0 a synthetic intermediate was separated into its two respective enantiomers by isocratic elution in around 7 minutes by reversed phase HPLC on an Astec Chirobiotic R column (250 x 4.6mm, Part No. 13024) using methanol and 0.1% TEAA in water, pH 4.1 (30:70). The enantiomers were detected by UV at 254nm.
  • N-CBZ Lysine by HPLC/UV Using Chirobiotic T Column
    N-CBZ Lysine, Astec Application Report 218 N-CBZ Lysine, CAS No. NA an amino acid derivative was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and water (50:50). The enantiomers were detected by UV at 210nm.
  • N-CBZ-Asparagine by HPLC/UV
    N-CBZ-Asparagine, Astec Application Report 203 N-CBZ-Asparagine, CAS No. NR, an amino acid was separated into its two respective enantiomers by isocratic elution (time not given) by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using methanol, water, at pH 4.1 with 1% TEAA (20:80). The enantiomers were detected by UV at 254nm.
  • N-CBZ-Valine by HPLC/UV Using Chirobiotic T Column
    N-CBZ-Valine, Astec Application Report 234 N-CBZ-Valine, CAS No. NA an amino acid derivative was separated into its two respective enantiomers by isocratic elution (no time given) by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using methanol and 1.0% TEAA in water, pH 4.1 (20:80). The enantiomers were detected by UV at 254nm.
  • N-FMOC Leucine by HPLC/UV Using Chirobiotic T Column
    N-FMOC Leucine, Astec Application Report 216 N-CBZ Lysine, CAS No. NA an amino acid derivative was separated into its two respective enantiomers by isocratic elution (no time reported) by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using methanol and water (60:40). The enantiomers were detected by UV at 254nm.
  • Naproxen by HPLC/UV Using Chirobiotic V Column
    Naproxen, Astec Application Report 133 Naproxen, CAS No. 22204-53-1 an anti-inflammatory drug was separated into its two respective enantiomers by isocratic elution in around 12 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using THF and 0.1% TEAA in water, pH 7 (10:90). The enantiomers were detected by UV at 254nm.
  • Nicardipine by HPLC/UV Using Chirobiotic V Column
    Nicardipine, Astec Application Report 155 Nicardipine, CAS No. 55985-32-5 an antianginal antihypertensive drug was separated into its two respective enantiomers by isocratic elution in around 11 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, acetic acid and TEA (100:0.02:0.01). The enantiomers were detected by UV at 254nm.
  • Norgestrel by HPLC/UV Using Cyclobond II Column
    Norgestrel, Astec Application Report 4017 Norgestrel, CAS No. 6533-00-2 a progestogen and oral contraceptive drug was separated into its two respective enantiomers by isocratic elution in around 19 minutes by reversed phase HPLC on an Astec Cyclobond II column (250 x 4.6mm, Part No. 41020) using acetonitrile and water (30:70). The enantiomers were detected by UV at 254nm.
  • Norketotifen by HPLC/UV Using Cyclobond I 2000 Column
    Norketotifen, Astec Application Report 4037 Norketotifen, CAS No. NA a metabolite of the antihistamine ketotifen was separated into its two respective enantiomers by isocratic elution in around 7 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 column (250 x 4.6mm, Part No. 20024) using acetonitrile and 10mM ammonium acetate in water, pH 3.8 (10:90). The enantiomers were detected by UV at 275nm.
  • Norleucine by HPLC/UV Using Chirobiotic T Column
    Norleucine, CAS No. 327-57-1 an amino acid was separated into its two respective enantiomers by isocratic elution in around 7 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and water (20:80). The enantiomers were detected by UV at 210nm.
  • Norleucine by HPLC/UV Using Chirobiotic Tag Column
    Norleucine, Astec Application Report 5024 Norleucine, CAS No. 327-57-1 an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol and water (30:70). The enantiomers were detected by UV at 220nm.
  • Normal Phase Separation of Six Steroids by HPLC
    Normal Phase Separation of Steroids, Grom Application Number 06087 A mixture of six steroids was separated using a GROM-SIL 120 Cyan-1 ST column (250 x 4mm, Part No. GSCN10512S2504) by isocratic elution with hexane, methylene chloride and 2-propanol 100/10/2 in around 20 minutes and detected by UV at 254nm. The steroids separated were progesterone, deoxycorticosterone, corticosterone, cortisone, prednisone and prednisolone.
  • Norphenylephrine by HPLC/UV Cyclobond I 2000 AC Column-B
    Norphenylephrine, Astec Application Report 4036 Norphenylephrine, CAS No. 4779-94-6 an adrenergic hormone was separated into its two respective enantiomers by isocratic elution in around 12 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 AC column (250 x 4.6mm, Part No. 20124) using methanol and 0.5% acetic acid in water, pH 5.5 (10:90). The enantiomers were detected by UV at 254nm.
  • Norphenylephrine by HPLC/UV Cyclobond I 2000 AC Column-A
    Norphenylephrine, Astec Application Report 4036 Norphenylephrine, CAS No. 4779-94-6 an adrenergic hormone was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 AC column (250 x 4.6mm, Part No. 20124) using methanol and 0.5% acetic acid in water, pH 5.5 (10:90). The enantiomers were detected by UV at 254nm.
  • Norvaline by HPLC/UV Using Chirobiotic T Column
    Norvaline, Astec Application Report 221 Norvaline, CAS No. 6600-40-4 an amino acid was separated into its two respective enantiomers by isocratic elution in around 6 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and water, (10:90). The enantiomers were detected by UV at 210nm.
  • Norvaline by HPLC/UV Using Chirobiotic Tag Column
    Norvaline, Astec Application Report 5025 Norvaline, CAS No. 6600-40-4 an amino acid was separated into its two respective enantiomers by isocratic elution in around 13 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol and water, (60:40). The enantiomers were detected by UV at 220nm.
  • o-chlorophenylalanine by HPLC/UV
    o-chlorophenylalanine, Astec Application Report 266 o-chlorophenylalanine, CAS No. NR, an amino acid was separated into its two respective enantiomers by isocratic elution in around 15 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol, water (30:70). The enantiomers were detected by UV at 210nm.
  • o-Tyrosine by HPLC/UV Using Chirobiotic T Column
    o-Tyrosine, Astec Application Report 229 o-Tyrosine, CAS No. 60-18-4 an amino acid was separated into its two respective enantiomers by isocratic elution in around 7 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and water (50:50). The enantiomers were detected by UV at 210nm.
  • o-Tyrosine by HPLC/UV Using Chirobiotic Tag Column
    o-Tyrosine, Astec Application Report 5032 o-Tyrosine, CAS No. 60-18-4 an amino acid was separated into its two respective enantiomers by isocratic elution in around 11 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol and water (60:40). The enantiomers were detected by UV at 230nm.
  • Organic Acids
    Organic Acids, Grom Application Number 01009 Glycolic acid, L-malic acid, malonic acid, lactic acid, acetic acid, maleic acid, citric acid, fumaric acid, succinic acid and propionic acid in a standard were separated using a GROM-SIL, 120 ODS-5 ST organic acids HPLC column (250 x 4mm, Part No. GSOD50512S2504) by isocratic elution with 20mM sodium phosphate at pH 2.8 in around 25 minutes and detected by UV at 220nm.
  • Organic Acids Standard
    Organic Acids Standard, Grom Application Number 10136 Five organic acids, tartaric acid, malic acid, lactic acid, acetic acid and citric acid, in a standard and in a white wine sample were separated using a GROM-SIL ODS organic acids HPLC column (250 x 4.6mm, Part No. GSAA205113S2504) by isocratic elution with 20mM sodium phosphate at pH 2.8 in around 12 minutes and detected by UV at 220nm.
  • Organic Acids Standard and White Wine Sample
    Organic Acids Standard and White Wine Sample, Grom Application Number 10137 Five organic acids, tartaric acid, malic acid, lactic acid, acetic acid and citric acid, in a standard and in a white wine sample were separated using a GROM-SIL ODS organic acids HPLC column (250 x 4.6mm, Part No. GSAA205113S2504) by isocratic elution with 20mM sodium phosphate at pH 2.8 in around 12 minutes and detected by UV at 220nm.
  • Ornithine by HPLC/UV Using Chirobiotic T Column
    Ornithine, Astec Application Report 222 Ornithine, CAS No. 70-26-8 an amino acid was separated into its two respective enantiomers by isocratic elution in around 9 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and water, (20:80). The enantiomers were detected by UV at 200nm.
  • Oxazepam by HPLC/UV Using Chirobiotic T Column-A
    Oxazepam, Astec Application Report 275 Oxazepam, CAS No. 605-75-1 an anxiolytic drug was separated into its two respective enantiomers by isocratic elution in around 5 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using 100% methanol. The enantiomers were detected by UV at 250nm.
  • Oxazepam by HPLC/UV Using Chirobiotic T Column-B
    Oxazepam, Astec Application Report 251 Oxazepam, CAS No. 605-75-1 an anxiolytic drug was separated into its two respective enantiomers by isocratic elution in around 5 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using methanol, acetic acid and TEA (100:0.1:0.1) The enantiomers were detected by UV at 254nm. On column racemization was prevented by lowering the column temperature to 15C.
  • Oxprenolol by HPLC/UV Using Chirobiotic T Column
    Oxprenolol, Astec Application Report 252 Oxprenolol, CAS No. 6452-71-7 an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 14 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using acetonitrile, methanol, acetic acid and TEA (55:45:0.3:0.2). The enantiomers were detected by UV at 230nm.
  • p-chlorophenylalanine by HPLC/UV
    o-chlorophenylalanine, Astec Application Report 259 p-chlorophenylalanine, CAS No. 7424-00-2, an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol, water (50:50). The enantiomers were detected by UV at 230nm.
  • p-Tyrosine by HPLC/UV Using Chirobiotic T Column
    p-Tyrosine, Astec Application Report 230 p-Tyrosine, CAS No. 60-18-4 an amino acid was separated into its two respective enantiomers by isocratic elution in around 7 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and water (50:50). The enantiomers were detected by UV at 210nm.
  • p-Tyrosine by HPLC/UV Using Chirobiotic Tag Column-A
    p-Tyrosine, Astec Application Report 5034 p-Tyrosine, CAS No. 60-18-4 an amino acid was separated into its two respective enantiomers by isocratic elution in around 17 minutes by polar organic mode HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol, acetic acid and TEA (100:0.1:0.1). The enantiomers were detected by UV at 230nm.
  • p-Tyrosine by HPLC/UV Using Chirobiotic Tag Column-B
    p-Tyrosine, Astec Application Report 5034 p-Tyrosine, CAS No. 60-18-4 an amino acid was separated into its two respective enantiomers by isocratic elution in around 9 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol, and water (30:70). The enantiomers were detected by UV at 230nm.
  • Penicillamine by HPLC/UV Using Chirobiotic T Column
    Penicillamine, Astec Application Report 267 Penicillamine, CAS No. 52-67-5 an anti-rheumatic drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and 0.1% TEAA in water, pH 3.8 (80:20). The enantiomers were detected by UV at 225nm.
  • Phensuximide by HPLC/UV Using Chirobiotic V Column
    Phensuximide, Astec Application Report 134 Phensuximide, CAS No. 86-34-0 an anticonvulsant drug was separated into its two respective enantiomers by isocratic elution in around 18 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using hexane and ethanol (70:30). The enantiomers were detected by UV at 254nm.
  • Phensuximide by HPLC/UV Using Cyclobond I 2000 DMP Column
    Phensuximide, Astec Application Report 4019 Phensuximide, CAS No. 86-34-0 an anticonvulsant drug was separated into its two respective enantiomers by isocratic elution in around 12 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 DMP column (250 x 4.6mm, Part No. 20724) using hexane and IPA (20:80). The enantiomers were detected by UV at 254nm.
  • Phenylalanine by HPLC/UV Using Chirobiotic T Column
    Phenylalanine, Astec Application Report 223 Phenylalanine, CAS No. 147-85-3 an amino acid was separated into its two respective enantiomers by isocratic elution in around 8 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and water (50:50). The enantiomers were detected by UV at 210nm.
  • Phenylalanine by HPLC/UV Using Chirobiotic Tag Column
    Phenylalanine, Astec Application Report 5027 Phenylalanine, CAS No. 147-85-3 an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol and water (60:40). The enantiomers were detected by UV at 230nm.
  • Phenylephrine by HPLC/UV Using Cyclobond I 2000 AC Column
    Phenylephrine, Astec Application Report 4020 Phenylephrine, CAS No. 61-76-7, an adrenergic agonist was separated into its two respective enantiomers by isocratic elution in around 15 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 AC column (250 x 4.6mm, Part No. 20124) using methanol and 0.5% sodium acetate in water, pH 5.5 (10:90). The enantiomers were detected by UV at 254nm.
  • Phenylglycine by HPLC/UV Using Chirobiotic Tag Column
    Phenylglycine, Astec Application Report 5028 Phenylglycine, CAS No. 875-74-1 an amino acid was separated into its two respective enantiomers by isocratic elution in around 32 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol and water (60:40). The enantiomers were detected by UV at 230nm.
  • Pindolol by HPLC/UV Using Chirobiotic T Column
    Pindolol, Astec Application Report 253 Pindolol, CAS No. 13523-86-9, an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 9 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using acetonitrile, methanol, acetic acid and TEA (55:45:0.3:0.2). The enantiomers were detected by UV at 230nm.
  • Pindolol by HPLC/UV Using Chirobiotic V Column
    Pindolol, Astec Application Report 135 Pindolol, CAS No. 13523-86-9, an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 11 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, acetic acid and TEA (100:0.01:0.01). The enantiomers were detected by UV at 254nm.
  • Pipecolic acid by HPLC/UV Using Chirobiotic Tag Column
    Pipecolic acid, Astec Application Report 5029 Pipecolic acid, CAS No. 535-75-1 an amino acid was separated into its two respective enantiomers by isocratic elution in around 16 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol and water (30:70). The enantiomers were detected by UV at 210nm.
  • Polycyclic Aromatic Hydrocarbons
    Polycyclic Aromatic Hydrocarbons, Grom Application Number 10163 Sixteen polycyclic aromatic hydrocarbons in a mixture were separated using a GROM-PAH HPLC column (50 x 4mm, Part No. GSPA10330S0504) by gradient elution with water, methanol 20/80 and 100% acetonitrile in around 5 minutes and detected by UV at 254nm. The compounds separated were naphthalene, acenaphthylene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, dibenzo(a,h)anthracene, benzo(g,h)perylene and indeno(1,2,3,c,d) pyrene.
  • Proglumide by HPLC/UV Using Chirobiotic V Column
    Proglumide, Astec Application Report 136 Proglumide, CAS No. 6620-60-6, an anti-holinergic drug was separated into its two respective enantiomers by isocratic elution in around 14 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using THF and 20 mM ammonium nitrate in water, pH 5.5 (10:90). The enantiomers were detected by UV at 254nm.
  • Promethazine by HPLC/UV Using Chirobiotic V Column
    Promethazine, Astec Application Report 137 Promethazine, CAS No. 60-87-7, an anti-nausea drug was separated into its two respective enantiomers by isocratic elution in around 16 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, acetic acid and TEA (100:0.1:0.1). The enantiomers were detected by UV at 240nm.
  • Promethazine sulfoxide by HPLC/UV Using Chirobiotic V Column
    Promethazine sulfoxide, Astec Application Report 138 Promethazine sulfoxide, CAS No. 7640-51-9 a metabolite of promethazine, an anti-nausea drug was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, acetic acid and TEA (100:0.1:0.1). The enantiomers were detected by UV at 240nm.
  • Propanolol by HPLC/UV Using Chirobiotic T Column-A
    Propanolol, Astec Application Report 276 Propanolol, CAS No. 525-66-6 an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 18 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using methanol and ammonium TFA (100:0.1w%). The enantiomers were detected by UV at 230nm.
  • Propanolol by HPLC/UV Using Chirobiotic T Column-B
    Propanolol, Astec Application Report 276 Propanolol, CAS No. 525-66-6 an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 18 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using methanol and ammonium TFA (100:0.1w%). The enantiomers were detected by UV at 230nm.
  • Propanolol by HPLC/UV Using Chirobiotic V Column
    Propanolol, Astec Application Report 139 Propanolol, CAS No. 525-66-6 an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 17 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, TFA and ammonium hydroxide (100:0.01:0.01). The enantiomers were detected by UV at 230nm.
  • Propanolol by HPLC/UV Using Cyclobond I 2000 Column
    Propanolol, Astec Application Report 4022 Propanolol, CAS No. 525-66-6 an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 24 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 column (250 x 4.6mm, Part No. 20024) using acetonitrile, methanol, acetic acid and TEA (95:5:0.3:0.2). The enantiomers were detected by UV at 230nm.
  • Protein Molecular Weight Determination by HPLC
    Protein Molecular Weight Determination, Grom Application Number 10185 A mixture of six proteins was separated using a Novarose SE-100/17 HPLC column (300 x 8mm, Part No. N101 290) by isocratic elution with 0.05 M sodium phosphate, pH 6.7, 0.15M sodium chloride in around 8 minutes and detected by UV at 214nm. The compounds separated were thyroglobulin, aldolase, ovalbumin, carbonic anhydrase, ribonuclease and insulin.
  • PTH-Amino Acid Separation by Gradient Elution HPLC
    PTH-Amino Acid Analysis by HPLC Applying Gradient Elution, Grom Application Number 10067 Nineteen amino acids in a standard mixture were separated using a NovoGROM column, Superspher C8 endcapped (250mm x 4mm, Part No. MSOC50406C2504) by gradient elution with 21mM sodium acetate, pH 4.9 and acetonitrile and 1,2-dichloromethane 993/7 in around 13 minutes and detected by UV at 254nm. The amino acids separated were aspartic acid, glutamic acid, asparagine, glutamine, serine, threonine, histidine, glycine, alanine, tyrosine, arginine, methionine, valine, proline, tryptophan, phenylalanine, isoleucine, lysine and leucine.
  • Pyridoglutethimide by HPLC/UV Using Chirobiotic V Column
    Pyridoglutethimide, Astec Application Report 140 Pyridoglutethimide, CAS No. 92788-10-8 an sedative drug was separated into its two respective enantiomers by isocratic elution in around 14 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using acetonitrile and 0.1% TEAA in water, pH 7.0 (10:90). The enantiomers were detected by UV at 254nm.
  • Pyroglutamic acid by HPLC/UV Using Chirobiotic T Column
    Pyroglutamic acid, Astec Application Report 225 Pyroglutamic acid, CAS No. 98-79-3 an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using acetonitrile, methanol, acetic acid and 0.1% TEA (55:45:0.3:0.2). The enantiomers were detected by UV at 254nm.
  • Regioisomeric Fullerene Derivatives by HPLC
    Regioisomeric Fullerene Derivatives, Grom Application Number 08008 A mixture of five regioisomeric fullerene derivatives was separated using a GROM-SIL 100 Normal PH-1 ST column (250 x 4mm, Part No. GSNP50510S2504) by isocratic elution with toluene in around 8 minutes and detected by UV at 340nm. The compounds separated were trans-1-C62(COOEt)4, trans-2-C62(COOEt)4, trans-3-C62(COOEt)4, trans-4-C62(COOEt)4 and e-C62(COOEt)4.
  • Ritalinic acid by HPLC/UV Using Chirobiotic T Column
    Ritalinic acid, Astec Application Report 277 Ritalinic acid, CAS No. NA a urinary metabolite of Ritalin was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using acetonitrile and 0.1% TEAA in water, pH 5.0 (50:50). The enantiomers were detected by UV at 230nm.
  • Rose Essence Separation by HPLC
    Rose Essence - Fingerprint, Grom Application Number 01050 A rose essence mixture was separated using a GROM-SIL 100 ODS-2 FE column (250 x 4mm, Part No. GSOD20510S2504) by gradient elution with water and acetonitrile in around 35 minutes and detected by UV at 254nm. The compounds separated were not identified.
  • Rosenta Separation by HPLC
    Rosenta Analysis by HPLC, Grom Application Number 01052 A rose essence mixture was separated using a GROM-SIL 100 ODS-2 FE column (250 x 4mm, Part No. GSOD20510S2504) by gradient elution with water and acetonitrile in around 35 minutes and detected by UV at 254nm. The compounds separated were not identified.
  • Ruelene by HPLC/UV Using Cyclobond I 2000 Column
    Ruelene, Astec Application Report 4038 Ruelene, CAS No. 299-86-5 a pesticide was separated into its two respective enantiomers by isocratic elution in around 8 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 column (250 x 4.6mm, Part No. 20024) using acetonitrile, acetic acid and TEA (100:0.2:0.1). The enantiomers were detected by UV at 254nm.
  • Saikosides Separation by HPLC
    HPLC Analysis of Saikosides, Grom Application Number 01096 A mixture of saikosides was separated using a GROM-SIL 120 ODS-3 CP column (250 x 4mm, Part No. GSOD30512S2504) by isocratic elution with water, methanol and acetonitrile 45/15/40 in around 28 minutes and detected by UV at 210nm. The compounds separated were saikosaponin c, saikosaponin f, saikosaponin a, saikosaponin b2, saikosaponin b1 and saikosaponin d.
  • Selegiline by HPLC/UV Using Cyclobond I 2000 DMP Column
    Selegiline, Astec Application Report 4039 Selegiline, CAS No. 14611-15-9 an anti-parkinsonian drug was separated into its two respective enantiomers by isocratic elution in around 8 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 DMP column (250 x 4.6mm, Part No. 20724) using acetonitrile and 20mM ammonium acetate in water, pH 4.1 (20:80). The enantiomers were detected by UV at 254nm.
  • Selegiline by HPLC/UV Using Cyclobond I 2000 SN Column
    Selegiline, Astec Application Report 4023 Selegiline, CAS No. 14611-15-9 an anti-parkinsonian drug was separated into its two respective enantiomers by isocratic elution in around 8 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 SN column (250 x 4.6mm, Part No. 20524) using acetonitrile and 1.0% TEAA in water, pH 4.1 (25:75). The enantiomers were detected by UV at 254nm.
  • Separation of Antineoplastics and Related Compounds
    Antineoplastics and Related Compounds, Grom Application Number 01006 A mixture of five antineoplastics and related compounds was separated using a GROM-SIL 120 ODS-5 ST column (150 x 4mm, Part No. GSOD50512S1504) by isocratic elution with 20mM ammonium phosphate in around 16 minutes and detected by UV at 254nm. The compounds separated were uracil, 5-fluorouracil, deoxyuridine and 5-fluoro-2-deoxyuridine.
  • Separation of Eight Mono and Disaccharides
    Separation of Eight Mono and Disaccharides, Grom Application Number 07123 A mixture of eight mono and disaccharides was separated using a GROM-SIL 80 Amino-3 CP column (250 x 4mm, Part No. GSNH30508S2504) by isocratic elution with water and acetontrile 15/85 in around 26 minutes and detected by UV at 254nm. The compounds separated were rhamnose, xylose, fructose, mannose, glucose, sucrose, lactose and maltose.
  • Separation of Eighteen Explosives Compounds by HPLC
    HPLC Analysis of Explosives, Grom Application Number 01128 A mixture of eighteen compounds found in explosives was separated using a GROM-SIL 80 ODS-7 PH column (250 x 4mm, Part No. GSOD70408S2504) by gradient elution with water and 0.3%THF v/v and methanol and 0.3%THF v/v in around 40 minutes and detected by UV at 240nm. The compounds separated were HMX, RDX, TNB, 2-am-4-NT, 1,2-DNB, DNB, 3,5-DNA, Tetryl, NB, TNT, 3,4-DNT, 4-Am-2,6-DNT, 2-Am-4,6-DNT, 2,6-DNT, 2,4-DNT, 2-NT, 4-NT and 3-NT.
  • Separation of Five Nucleotides by HPLC
    Separation of Nucleotides by HPLC, Grom Application Number 07044 A mixture of five nucleotides was separated using a GROM-SIL 120 Amino-2 PA HPLC column (250 x 4mm, Part No. GSMH20512S2504) by isocratic elution with potassium biphosphate in around 25 minutes and detected by UV at 260nm. The compounds separated were 5-CMP, 5-AMP, 5-UMP, 5-IMP and 5-GMP.
  • Separation of Five Polyphenols by HPLC
    Polyphenols-1, Grom Application Number 01019 A mixture of five polyphenols was separated using a GROM-SIL 120 ODS-5 ST column (250 x 4mm, Part No. GSOD50512S2504) by isocratic elution with 50mM phosphoric acid and acetonitrile 90/10 in around 30 minutes and detected by UV at 280nm. The polyphenols separated were phloroglucinol, hydroquinone, resorcinol, catechol and phenol.
  • Separation of Five Proteins by Ion Exchange HPLC
    Separation of Proteins by Ion Exchange HPLC, Grom Application Number 09105 A mixture of five proteins was separated using a GROM-SIL 300 WCX ion exchange column (250 x 4mm, Part No. GSWC10730S2505) by gradient elution with 0.05M sodium phosphate, pH 6.4 and 0.5M sodium phosphate, pH 6.4 in around 50 minutes and detected by UV at 280nm. The proteins separated were trypsinogen, ribonuclease A, cytochrome C, chymotrypsinogen and lysozyme.
  • Separation of Five Steroids by HPLC
    HPLC of Steroids, Grom Application Number 06124 A mixture of five steroids was separated using a GROM-SIL 120 Cyan-3 CP column (250 x 4mm, Part No. GSOD30512S2504) by isocratic elution with ethanol and n-hexane 5/95 in around 12 minutes and detected by UV at 220nm. The steroids separated were progesterone, estrone, estradiol, ethynylestradiol and prednisolone.
  • Separation of Five Water Soluble Vitamins
    Water-Soluble Vitamins, Grom Application Number 01015 A mixture of five water-soluble vitamins was separated using a GROM-SIL 120 ODS-5 ST column (150 x 4mm, Part No. GSOD50512S1504) by isocratic elution with 20mM sodium phosphate in around 20 minutes and detected by UV at 210nm. The compounds separated were nicotinic acid, pantothenic acid, pyridoxine hydrochloride (Vitamin B6), nicotinamide and thiamine hydrochloride (Vitamin B1).
  • Separation of Four Antibiotics
    Analysis of Antibiotics, Grom Application Number 01114 A mixture of nine antibiotic drugs was separated using a GROM-SIL 120 ODS-4 HE column (125 x 4mm, Part No. GSOD40512S1204) by isocratic elution with 1% triethylammonium acetate pH 4.0 and acetonitrile 70/30 in around 10 minutes and detected by UV at 220nm. The compounds separated were ampicillin, oxacillin, coxacillin and dicloxacillin.
  • Separation of Four Corticosteroids by HPLC
    HPLC Analysis of Corticosteroids, Grom Application Number 01090 A mixture of four corticosteroids was separated using a GROM-SIL 120 ODS-4 HE column (250 x 4mm, Part No. GSOD40512S2504) by isocratic elution with water and methanol 40/60 in around 20 minutes and detected by UV at 230nm. The corticosteroids separated were cortisone, hydroxycortisone, corticosterone and cortisone acetate.
  • Separation of Four Polyphenols by HPLC
    Analysis of Polyphenols II, Grom Application Number 03040 A mixture of four polyphenols was separated using a GROM-SIL 120 Phenyl-1 FE column (250 x 4mm, Part No. GSPH10512S2504) by isocratic elution with 5mM acetic acid in around 16 minutes and detected by UV at 280nm. The polyphenols separated were pyrogallol, hydroquinone, catechol and phenol.
  • Separation of Insulins from Different Species by HPLC
    Insulins from Different Species, Grom Application Number 01018 A mixture of four insulins from different species was separated using a GROM-SIL 120 ODS-5 ST column (150 x 4mm, Part No. GSOD50512S1504) by gradient elution with 0.01N HCl and acetonitrile 72/28 and 0.01N HCl and acetonitrile 65/35 in around 20 minutes and detected by UV at 220nm. The compounds separated were insulin (bovine), insulin (sheep), insulin (human) and insulin (porcine).
  • Separation of n-Alkyl Benzenes Using Zirconia Phases
    N-Alkyl Benzenes, Zirconia Phases, Grom Application Number 10174 Seven n-alkyl benzene compounds in a standard were separated using a GROM-Zirconia RP P column (50 x 2mm, Part No. GZOD10330S0502) by gradient elution with water and acetonitrile 30/70 in around 3 minutes and detected by UV at 254nm. The n-alkyl benzenes separated were benzene, toluene, ethylbenzene, propylbenzene, butylbenzene, pentylbenzene and hexylbenzene.
  • Separation of Nonylphenylethoxylates-Nonionic Surfactants by HPL
    Nonylphenylethoxylates-Nonionic Surfactants, Grom Application Number 08049 The nonionic surfactant Nonylphenol-10 EO, a nonylphenylethoxylate was separated using a GROM-SIL 100 Norm PH-1 ST column (250 x 4mm, Part No. GSNP10510S2504) by gradient elution with water and ethyl acetate 1/99 and water and THF 5/95 in around 25 minutes and detected by UV at 254nm. The compounds separated were not identified.
  • Separation of PAH’s by Gradient Elution
    Separation of PAHs by Gradient Elution, Grom Application Number 10037 Sixteen polycyclic aromatic hydrocarbons in a mixture were separated using a GROM-SIL PAH-1 HPLC column (250 x 4mm, Part No. GSPA10530S2504) by gradient elution with water and methanol in around 25 minutes and detected by UV at 254nm. The compounds separated were naphthalene, acenaphthylene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, dibenzo(a,h)anthracene, benzo(g,h)perylene and indeno(1,2,3,c,d) pyrene.
  • Separation of Peptides by HPLC
    Peptides, Grom Application Number 10188 A mixture of peptides was separated using a GROM-SIL 100 ODS-2 FE column (33 x 0.3mm, Part No. GSOD20210C0330) by gradient elution with 0.1% TFA in water and acetonitrile and 0.1% TFA in around 18 minutes and detected by UV at 214nm. The compounds separated were not identified.
  • Separation of Poly(A)Oligomers by HPLC
    Poly(A)Oligomers, Grom Application Number 01017 A mixture of poly(a)oligomers was separated using a GROM-SIL 120 ODS-5 ST column (150 x 4mm, Part No. GSOD50512S1504) by gradient elution with 0.1M sodium phosphate, pH 7.0 and 0.1M sodium phosphate, pH 7.0 and acetonitrile 75/25 in around 60 minutes and detected by UV at 260nm. The compounds separated were not identified.
  • Separation of Protein Digest by HPLC
    Protein Digest, Grom Application Number 10182 A protein digest was separated using a GROM-SIL 100 ODS-2 FE column (33 x 2.0mm, Part No. GSOD20210S0405) by gradient elution with 0.1% TFA in water and acetonitrile and 0.1% TFA in around 14 minutes and detected by UV at 214nm. The compounds separated were not identified.
  • Separation of Seven Steroids by HPLC
    Steroid Separation by HPLC, Grom Application Number 01092 A mixture of seven steroids was separated using a GROM-SIL 120 ODS-3 CP column (250 x 4mm, Part No. GSOD30512S2504) by isocratic elution with water, methanol and acetonitrile 10/40/23 in around 13 minutes and detected by UV at 243nm. The steroids separated were predonisolone, fluocinolone acetonide, hydrocortisone acetate, dexamethasone acetate, hydrocortisone butylate, fluocinonide and clobetazol propionate.
  • Separation of Six Nucleotides by HPLC
    Separation of Nucleotides by HPLC, Grom Application Number 07127 A mixture of five nucleotides was separated using a GROM-SIL 80 Amino-3 CP HPLC column (250 x 4.6mm, Part No. GSNH30508S2504) by gradient elution with 5mM potassium biphosphate, pH 2.9 and 15% acetonitrile and 500mM potassium biphosphate, pH 4.0 and 15% acetonitrile in around 25 minutes and detected by UV at 254nm. The compounds separated were GMP, AMP, IMP, GDP, ADP and IDP.
  • Separation of Six Peptides
    Peptide Separation, Grom Application Number 10129 A mixture of six peptides was separated using a GROM-BIO RP-1 (C18) column (250 x 2mm, Part No. GSBR10530S2502) by gradient elution with 0.1% TFA in water and 0.1% TFA inwater and acetonitrile 30/70 in around 20 minutes and detected by UV at 210nm. The compounds separated were oxytocin, bradykinin, angiotensin, eledoisin, neurotensin and angiotensin I.
  • Separation of Ten Fat Soluble Vitamins
    Fat-Soluble Vitamins, Grom Application Number 01003 A mixture of ten fat-soluble vitamins was separated using a GROM-SIL 120 ODS-5 ST column (150 x 4mm, Part No. GSOD50512S1504) by isocratic elution with acetontrile in around 22 minutes and detected by UV at 280nm. The compounds separated were menadione (Vitamin K3), retinol (Vitamin A), retinal acetate, menaquinone (Vitamin K2), d-tocopherol, ergocalciferol (Vitamin D2), cholecalciferol (Vitamin D3), a-tocopherol (Vitamin E), a-tocopherol acetate and phylloquinone (Vitamin K1).
  • Separation of Three Food Preservatives by HPLC
    Analysis of Food Preservatives, Grom Application Number 01081 A mixture of three food preservatives was separated using a GROM-SIL 120 ODS-3 CP column (125 x 4mm, Part No. GSOD30512S1204) by isocratic elution with water and acetonitrile 50/50 v/v in around 4 minutes and detected by UV at 254nm. The compounds separated were p-hydroxybenzoic acid, methylhydroxybenzoic acid and propylhydroxybenzoic acid.
  • Separation of Three Nonsteroidal Anti-inflammatory Drugs
    Nonsteroid Anti-inflammatory Drugs, Grom Application Number 01031a Three nonsteroidal anti-inflammatory drugs were separated using a GROM-SIL 100 ODS-2 FE column (250 x 4mm, Part No. GSOD20510S2504) by isocratic elution with acetonitrile and 0.1% TFA in water 35/65 in around 43 minutes and detected by UV at 254nm. The compounds separated were ketoprofen, diclofenac and ibuprofen.
  • Separation of TNT Isomers by HPLC
    Analysis of Trinitrotoluene Isomers, Grom Application Number 08076 A mixture of six TNT (trinitrotoluene) isomers was separated using a GROM-SIL 100 Normal PH-1 ST column (125 x 4mm, Part No. GSNP10310S120404) by isocratic elution with n-hexane and methylene chloride 87.5/12.5 in around 18 minutes and detected by UV at 254nm. The compounds separated were 2,4,6-TNT, 2,3,6-TNT, 2,3,5-TNT, 2,4,5-TNT, 2,3,4-TNT and 3,4,5-TNT.
  • Separation Using Zirconia Phases
    Resorcinol, Benzonitrile, Methyl Benzoate, Anisole, Zirconia Phases, Grom Application Number 10173 A mixture of four compounds (resorcinol, benzonitrile, methyl benzoate and anisole) was separated using a GROM-Zirconia RP P column (150 x 4.6mm, Part No. GZOD10330S1505) by isocratic elution with water and acetonitrile 85/15 in around 11 minutes and detected by UV at 254nm.
  • Serine by HPLC/UV Using Chirobiotic T Column
    Serine, Astec Application Report 226 Serine, CAS No. 56-45-1 an amino acid was separated into its two respective enantiomers by isocratic elution in around 7 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and water (30:70). The enantiomers were detected by UV at 200nm.
  • Serine by HPLC/UV Using Chirobiotic Tag Column
    Serine, Astec Application Report 5030 Serine, CAS No. 56-45-1 an amino acid was separated into its two respective enantiomers by isocratic elution in around 5 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol and water (30:70). The enantiomers were detected by UV at 210nm.
  • Sertraline by HPLC/UV Using Cyclobond I 2000 RSP Column
    Sertraline, Astec Application Report 4040 Sertraline, CAS No. 79617-96-2 an antidepressant was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 RSP column (250 x 4.6mm, Part No. 20324) using acetonitrile and 20mM ammonium acetate in water, pH 4.1 (30:70). The enantiomers were detected by UV at 254nm.
  • Six Ingredients in a Cough/Cold Medication by HPLC
    Ingredients in a Cough/Cold Medication, Grom Application Number 01014 A mixture of six ingredients in a cough/cold medication was separated using a GROM-SIL 120 ODS-5 ST column (250 x 4mm, Part No. GSOD50512S2504) by isocratic elution with 0.1% phosphoric acid, methanol and acetonitrile 77/15/18 in around 17 minutes and detected by UV at 245nm. The ingredients separated were acetaminophen, caffeine, chloropheniramine malate, narcotine, ethenzamide and brucetine.
  • Six Peptides and Proteins by HPLC
    Peptides and Proteins, Grom Application Number 01002 A mixture of six peptides and proteins were separated using a GROM-SIL 120 ODS-5 ST column (150 x 4mm, Part No. GSOD50512S000) by gradient elution with acetonitrile, water and TFA 20/80/0.05 and acetonitrile, water and TFA 45/55/0.05 in around 21 minutes and detected by UV at 220nm. The proteins and peptides separated were met-enkephalin, leu-enkephalin, bovine serum albumin, a-mating-factor, insulin and ovalbumin.
  • Sotalol by HPLC/UV Using Chirobiotic T Column
    Sotalol, Astec Application Report 255 Sotalol, CAS No. 3930-20-9 an anti-anginal drug was separated into its two respective enantiomers by isocratic elution in around 11 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using acetonitrile, methanol acetic acid and TEA (55:45:0.3:0.2). The enantiomers were detected by UV at 230nm.
  • Sotalol by HPLC/UV Using Chirobiotic V Column
    Sotalol, Astec Application Report 141 Sotalol, CAS No. 3930-20-9 an anti-anginal drug was separated into its two respective enantiomers by isocratic elution in around 21 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, acetic acid and TEA (100:0.01:0.01). The enantiomers were detected by UV at 230nm.
  • Steric Exclusion Chromatography of Peptides
    Steric Exclusion Chromatography of Peptides, Grom Application Number 09045b A mixture of three peptides was separated using a GROM-SIL 120 SEC column (500 x 8mm, Part No. GSSE10512S5008) by isocratic elution with 0.1M potassium phosphate, 0,2 M sodium chloride, pH 7.0 and acetonitrile 70.30 in around 60 minutes and detected by UV at 215nm. The compounds separated were insulin (bovine) 2 neurotensin., angiotensin-II and glycine.
  • Steric Exclusion Chromatography of Peptides –1
    Steric Exclusion Chromatography of Peptides 1, Grom Application Number 09045a A mixture of three peptides and an amino acid was separated using a GROM-SIL 60 SEC column (500 x 8mm, Part No. GSEE10506S5008) by isocratic elution with 0.1M potassium phosphate, 0.2M sodium chloride, pH 7.0 and acetonitrile 70/30 in around 30 minutes and detected by UV at 215nm. The compounds separated were insulin (bovine), neurotensin, angiotensin-II and glycine.
  • Steric Exclusion Chromatography of Proteins-I
    Steric Exclusion Chromatography of Proteins-I, Grom Application Number 09046a Five proteins were separated using a GROM-SIL 120 SEC column (500 x 8mm, Part No. GSEE10512S5008) by isocratic elution with 0.1M potassium phosphate, 0.2M sodium chloride, pH 7.0 and acetonitrile 70/30 in around 16 minutes and detected by UV at 280nm. The compounds separated were glutamate dehydrogenase (MW 290,000), lactate dehydrogenase (MW 142,000), enolase (MW 67,000), adenylate kinase (MW 32,000) and cytochrome C (MW 12,000).
  • Steric Exclusion Chromatography of Proteins-II
    Steric Exclusion Chromatography of Proteins-II, Grom Application Number 09046b A mixture of five proteins was separated using a GROM-SIL 200 SEC HPLC column (500 x 8mm, Part No. GSSE10520S5008) by isocratic elution with 0.1M potassium phosphate (0.2M sodium chloride, pH 7.0) and acetonitrile 70/30 in around 50 minutes and detected by UV at 280nm. The compounds separated were glutamate dehydrogenase (MW 290,000), lactate dehydrogenase (MW 142,000), enolase (MW 67,000), adenylate kinase (MW 32,000) and cytochrome C (MW 12,000).
  • Strigol by HPLC/UV Using Cyclobond I 2000 RSP Column
    Strigol, Astec Application Report 4024 Strigol, CAS No. 11017-56-4, a seed germination stimulant was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 RSP column (250 x 4.6mm, Part No. 20324) using acetonitrile and 1.0% TEAA in water, pH 4.1 (25:75). The enantiomers were detected by UV at 240nm.
  • Sugar Nucleotides by HPLC
    Sugar Nucleotides by HPLC, Grom Application Number 01024 A mixture of five sugar nucleotides was separated using a GROM-SIL 120 ODS-5 ST HPLC column (250 x 4mm, Part No. GSOD50512S2504) by isocratic elution with 20 mM triethylammonium acetate, pH 5.7 and acetonitrile 99/1 in around 25 minutes and detected by UV at 260nm. The compounds separated were CDP-d glucose, UDP-d-glucose, UDP-n-acetyl-d-glucosamine, GDP-d-mannose and ADP-d-glucose.
  • Sulfonamides Separation by HPLC
    Analysis of Sulfonamides, Grom Application Number 01112 A mixture of sulfonamides was separated using a GROM-SIL 100 ODS-2 FE column (250 x 4mm, Part No. GSOD20510S2504) by isocratic elution with water and methanol 60/40 in around 11 minutes and detected by UV at 230nm. The compounds separated were sulfaguanidine, sulfathiazole, sulfapyridine and sulfamethazine.
  • Sulfur Containing Native Amino Acids by HPLC
    Sulfur Containing Native Amino Acids, Grom Application Number 01028 A mixture of three sulfur-containing amino acids (cystine, cysteine and methionine) was separated using a GROM-SIL 120 ODS-4 HE HPLC column (250 x 4mm, Part No. GSOD40512S2504) by isocratic elution with water in around 7 minutes and detected by UV at 210nm.
  • Ten Preservatives by HPLC
    Analysis of Preservatives, Grom Application Number 01110 Ten preservatives in a standard were separated using a GROM-SIL 100 ODS-3 CP column (250 x 4mm, Part No. GSOD30512S2504) by isocratic elution with 0.05M sodium phosphate, pH 4.5, 4mM cetyltrimethylammonium chloride; methanol and acetonitrile 50/35/15 in around 12 minutes and detected by UV at 235nm. The preservatives separated were methylparaben, p-hydroxybenzoic acid, ethylparaben, dehydroacetic acid, n-propylparaben, sorbic acid, benzoic acid, isobutylparaben, n-butylparaben and salicylic acid.
  • Terbutaline by HPLC/UV Using Chirobiotic T Column-A
    Terbutaline, Astec Application Report 262 Terbutaline, CAS No. 23031-25-6 a bronchodilator drug was separated into its two respective enantiomers by isocratic elution in around 12 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using methanol, acetic acid and TEA (100:0.2:0.1). The enantiomers were detected by UV at 254nm.
  • Terbutaline by HPLC/UV Using Chirobiotic T Column-B
    Terbutaline, Astec Application Report 278 Terbutaline, CAS No. 23031-25-6 a bronchodilator drug was separated into its two respective enantiomers by isocratic elution in around 14 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using methanol and ammonium TFA (100:0.1w%). The enantiomers were detected by UV at 230nm.
  • Terbutaline by HPLC/UV Using Chirobiotic V Column
    Terbutaline, Astec Application Report 142 Terbutaline, CAS No. 23031-25-6 a bronchodilator drug was separated into its two respective enantiomers by isocratic elution in around 13 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol TFA and ammonium hydroxide (100:0.01:0.01). The enantiomers were detected by UV at 230nm.
  • Thalidomide by HPLC/UV Using Chirobiotic V Column
    Thalidomide, Astec Application Report 151 Thalidomide, CAS No. 50-35-1 an immunomodulator drug was separated into its two respective enantiomers by isocratic elution in around 6 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using acetonitrile and 18mM ammonium nitrate in water (35:65). The enantiomers were detected by UV at 254nm.
  • Three Cardiac Glycosides by HPLC
    HPLC of Cardiac Glycosides, Grom Application Number 02089 A mixture of three cardiac glycosides was separated using a GROM-SIL 120 Octyl-5 CP column (250 x 4mm, Part No. GSOC50512S2504) by isocratic elution with water and acetonitrile 60/40 in around 8 minutes and detected by UV at 220nm. The glycosides separated were esculin, strophantin and digitoxin.
  • Three Water Soluble Vitamins Separated by HPLC
    Water-Soluble Vitamins, Grom Application Number 07043 Three water-soluble vitamins in a standard were separated using a GROM-SIL 120 Amino-2 PA column (250 x 4.6mm, Part No. GSNH20512S2504) by isocratic elution with 50mM ammonium phosphate and acetonitrile 30/70 in around 12 minutes and detected by UV at 254nm. The vitamins separated were nicotinic acid, isoascorbic acid and L-ascorbic acid.
  • Threonine by HPLC/UV Using Chirobiotic T Column
    Threonine, Astec Application Report 227 Threonine, CAS No. 72-19-5 an amino acid was separated into its two respective enantiomers by isocratic elution in around 7 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and water (70:30). The enantiomers were detected by UV at 200nm.
  • Tocopherols Separation by HPLC
    Analysis of Tocopherols, Grom Application Number 07042 A mixture of tocopherols was separated using a GROM-SIL 120 Amino-2 PA column (250 x 4mm, Part No. GSNH20512S2504) by isocratic elution with hexane and ethyl acetate 70/30 in around 30 minutes and detected by UV at 295nm. The compounds separated were a-tocopherol, b-tocopherol, g-tocopherol and d-tocopherol.
  • Tramadol by HPLC/UV Using Cyclobond I 2000 DMP Column
    Tramadol, Astec Application Report 4025 Tramadol, CAS No. 27203-92-5 an analgesic drug was separated into its two respective enantiomers by isocratic elution in around 7 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 DMP column (250 x 4.6mm, Part No. 20724) using acetonitrile and 0.1% TEAA in water, pH 5.0 (20:80). The enantiomers were detected by UV at 254nm.
  • Trimipramine by HPLC/UV Using Chirobiotic V Column
    Trimipramine, Astec Application Report 153 Trimipramine, CAS No. 739-71-9 an antidepressant drug was separated into its two respective enantiomers by isocratic elution in around 19 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, acetic acid and TEA (100:0.03:0.02). The enantiomers were detected by UV at 230nm.
  • Tryptic Digest of Lactic Dehydrogenase
    Tryptic Digest of Lactic Dehydrogenase (carboxymethylated), Grom Application Number 10179 A carboxymethylated tryptic digest of lactic dehydrogenase was separated using a GROM-SIL 100 ODS-2 FE column (32 x 4.6mm, Part No. GSOD20210S0405) by gradient elution with water and 0.1% TFA and acetonitrile and 0.1% TFA in around 13 minutes and detected by UV at 215nm. No individual compounds were identified.
  • Tryptophan by HPLC/UV Using Chirobiotic T Column
    Tryptrophan, Astec Application Report 231 Tryptophan, CAS No. 73-22-3 an amino acid was separated into its two respective enantiomers by isocratic elution in around 8 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and water (30:70). The enantiomers were detected by UV at 210nm.
  • Twelve Organic Acids by HPLC
    Fatty Acid Analysis by HPLC p-Bromophenacylesters of Fatty Acids, Grom Application Number 01099 A mixture of twelve organic acids was separated using a GROM-SIL 100 ODS-0 AB column (250 x 4mm, Part No. GSOD00510S2504) by gradient elution with phosphoric acid, water and acetonitrile 1/70/30 and acetonitrile in around 50 minutes and detected by UV at 254nm. The compounds separated were pentanoic acid, hexanoic acid, heptanoic acid, octanoic acid, nonanoic acid, decanoic acid, dodecanoic acid, tertradecanoic acid, hexadecanoic acid, octadecanoic acid, eicosanoic acid and docasanoic acid. The title of the application note appears to be incorrect.
  • Two Benzodiazepin Sedatives by HPLC
    Sedativa - Benzodiazepins, Grom Application Number 10130a Two benzodiazepin sedatives in a standard were separated using a GROM-SIL 100 ODS-2 FE column (250 x 4mm, Part No. GSOD20510S2504) by isocratic elution with acetonitrile and 0.1% TFA in water 35/65 in around 18 minutes and detected by UV at 254nm. The benzodiazepins separated were clonazepam and diazepam along with the preservative benzyl alcohol.
  • Two Categories of Hop Bitter Acids in Beer
    Hop Bitter Acid Analysis in Beer by HPLC, Grom Application Number 10101 Two categories of hop bitter acids in a beer sample were separated using a GROM-Beer-Bitter column (125 x 4mm, Part No. GSBI1071S1204) by gradient elution with 0.1% phosphoric acid in water and acetonitrile in around 6 minutes and detected by UV at 280nm. The hop bitter acids separated were b-acids and iso-a-acids.
  • Two Sweeteners in Beverages by HPLC
    Sweeteners in Beverages, Grom Application Number 01074 Two beverage sweeteners in a standard were separated using a GROM-SIL 100 ODS-2 FE column (125 x 3mm, Part No. GSOD20510S1204) by isocratic elution with 12mM TBAH, 0.2M sodium phosphate, pH 3.6, water and acetonitrile 32/60/8 in around 12 minutes and detected by UV at 230nm. The sweeteners separated were sorbic acid and saccharin along with the preservative benzoic acid.
  • Valine by HPLC/UV Using Chirobiotic T Column
    Valine, Astec Application Report 233 Valine, CAS No. 72-18-4-1 an amino acid was separated into its two respective enantiomers by isocratic elution in around 7 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol and water (50:50). The enantiomers were detected by UV at 210nm.
  • Valine by HPLC/UV Using Chirobiotic Tag Column
    Valine, Astec Application Report 5036 Valine, CAS No. 72-18-4-1 an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol and water (60:40). The enantiomers were detected by UV at 210nm.
  • Warfarin by HPLC/UV Using Chirobiotic V Column
    Warfarin, Astec Application Report 144 Warfarin, CAS No. 81-81-2 an anticoagulant/rodenticide was separated into its two respective enantiomers by isocratic elution in around 13 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using THF and 0.1% TEAA in water, pH 5 (20:80). The enantiomers were detected by UV at 254nm.
  • Warfarin by HPLC/UV Using Cyclobond I 2000 Column-A
    Warfarin, Astec Application Report 4026 Warfarin, CAS No. 81-81-2 an anticoagulant/rodenticide was separated into its two respective enantiomers by isocratic elution in around 12 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 column (250 x 4.6mm, Part No. 20024) using acetonitrile, methanol, acetic acid and TEA (95:5:0.3:0.2). The enantiomers were detected by UV at 254nm.
  • Warfarin by HPLC/UV Using Cyclobond I 2000 Column-B
    Warfarin, Astec Application Report 4041 Warfarin, CAS No. 81-81-2 an anticoagulant/rodenticide was separated into its two respective enantiomers by isocratic elution in around 9 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 column (250 x 4.6mm, Part No. 20024) using acetonitrile, acetic acid and TEA (100:0.3:0.2). The enantiomers were detected by UV at 254nm.
  • Xylole Isomers by HPLC
    Xylole Isomers, Grom Application Number 01138 A mixture of three xylole isomers was separated using a GROM-SIL 60 ODS-5 ST column (250 x 4mm, Part No. GSOD50506S2504) by isocratic elution with water and acetontrile 40/60 in around 17 minutes and detected by UV at 254nm. The compounds separated were o-xylole, p-xylole and m-xylole.