HPLC/Fluorescence

HPLC separation with fluorescence detection.

Contents

  • Amino Acid Analysis After Precolumn Derivatization
    Amino Acid Analysis after Precolumn Derivatisation with OPA/Mercaptopropionic Acid Extended Standard, Grom Application Number 10062 Nineteen amino acids in a standard mixture were separated using a GROM-SIL OPA-1 column (150 x 4mm, Part No. GSOP10308S1504) after precolumn derivatization with OPA/mercaptopropionic acid by gradient elution with 25mM sodium phosphate, pH 7.2 and THF 995/5 and 25mM sodium phosphate, pH 7.2, methanol and acetonitrile 50/35/15 in around 41 minutes and detected by fluorescence spectroscopy at 330 and 450nm. The amino acids separated were aspartic acid, glutamic acid, asparagine, serine, glutamine, glycine, threonine, histidine, citrulline, 3-methylhistidine, alanine, taurine, arginine, alpha-aminobutyric acid, tyrosine, valine, methionine, nor-valine and tryptophan.
  • Amino Acid Analysis by Precolumn Derivatization with FMOC
    Amino Acid Analysis Employing Precolumn Derivatisation with FMOC Followed by Pentane Extraction, Grom Application Number 10130 Eighteen amino acids in a standard were separated using a GROM-SIL FMOC-1 column (250 x 4.6mm, Part No. GSFM10410S2505) after precolumn derivatization with FMOC (followed by pentane extraction) by gradient elution with 50mM sodium acetate, pH 4.2 and acetonitrile 80/20 and 50mM sodium acetate, pH 4.2 and acetonitrile 20/80 in around 45 minutes and detected by fluorescence spectroscopy at 263 and 313nm. The amino acids separated were cysteine sulphinic acid, aspartic acid, serine, glutamic acid, threonine, glycine, arginine, alanine, tyrosine, proline, methionine, valine, nor-valine, phenylalanine, isoleucine, leucine, histidine and lysine.
  • Amino Acid Analysis of Human Urodilatin
    Amino Acid Analysis of Human Urodilatin (hydrol. with 6N HCl, 15 hrs), Grom Application Number 10057 Amino acids in human urodilatin were separated after hydrolysis (6N HCl for 15 hours) using a GROM-SIL 120 ODS-5 ST column (150 x 4mm, Part No. GSFM20512S2504) by gradient elution with 50mM sodium acetate, pH 4.2 and acetonitrile 80/20 and 50mM sodium acetate, pH 4.2 and acetonitrile 20/80 in around 48 minutes and detected by by fluorescence spectroscopy at 263 and 313nm. The compounds separated were arginine, serine, aspartic acid, glutamic acid, threonine, glycine, alanine, tyrosine, proline, FMOH, ammonia, methionine, phenylalanine, isoleucine, leucine, histidine and lysine.
  • Amino Acid Analysis of Liver Biopsy Sample
    Amino Acid Analysis of Liver Biopsy Sample Using OPA for Precolumn Derivatisation, Grom Application Number 10061 Fourteen amino acids in a liver biopsy sample were separated using a GROM-SIL OPA-1 column (125 x 4mm, Part No. GSOP10308S1204) after precolumn derivatization with OPA by gradient elution with 12.5mM sodium phosphate, pH 7.2 and acetonitrile 97/2 and 12.5mM sodium phosphate, pH 7.2 and acetonitrile 50/50 in around 14 minutes and detected by fluorescence spectroscopy at 330 and 450nm. The amino acids separated were glutamic acid, alpha-aminoadipic acid, serine, glutamine, glycine, histidine, citrulline, 3-methylhistidine, arginine, tyrosine, valine, nor-valine, isoleucine, phenylalanine, leucine and lysine.
  • Amino Acids in Bovine Serum Albumin Hydrolysate
    Amino Acid Analysis of Bovine Serum Albumin Hydrolysate (6N HCl/15 hours), Grom Application Number 10056 Amino acids in a bovine serum albumin hydrolysate (6N HCl/15 hours) were separated using a GROM-SIL FMOC-2 column (250 x 4mm, Part No. GSFM20512S2504) by gradient elution with 50 mM sodium acetate and acetonitrile 80/20 and 50 mM sodium acetate/acetonitrile 20/80 at pH 4.2 in around 50 minutes and detected by fluorescence at 263 and 313nm. The amino acids and other compounds separated were arginine, serine, aspartic acid, glutamic acid, threonine, glycine, alanine, tyrosine, proline, FMOC-OH, methionine, valine, phenylalanine, isoleucine, leucine, histidine and lysine.
  • Amino Acids in Human Urodilatin Hydrolysate
    Amino Acid Analysis of Human Urodilatin Hydrolysate (6N HCl/15 hours), Grom Application Number 10057 Amino acids in human urodilatin hydrolysate (6N HCl/15 hours) were separated using a GROM-SIL FMOC-2 column (250 x 4mm, Part No. GSFM20512S2504) by gradient elution with 50 mM sodium acetate and acetonitrile 80/20 and 50 mM sodium acetate/acetonitrile 20/80 at pH 4.2 in around 50 minutes and detected by fluorescence at 263 and 313nm. The amino acids and other compounds separated were arginine, serine, aspartic acid, glutamic acid, threonine, glycine, alanine, tyrosine, proline, FMOC-OH, ammonia, methionine, phenylalanine, isoleucine, leucine, histidine and lysine.
  • Amino Acids in Insulin Hydrolysate
    Amino Acid Analysis- B-Chain of Insulin (6N HCl/15 hours), Grom Application Number 10055 Amino acids in a protein hydrolysate of the B-chain of insulin (6N HCl/15 hours) were separated using a GROM-SIL FMOC-2 column (250 x 4mm, Part No. GSFM20512S2504) by gradient elution with 50 mM sodium acetate and acetonitrile 80/20 and 50 mM sodium acetate/acetonitrile 20/80 at pH 4.2 in around 50 minutes and detected by fluorescence at 263 and 313nm. The amino acids and other compounds separated were cysteine sulphinic acid, arginine, serine, aspartic acid, glutamic acid, threonine, glycine, alanine, tyrosine, proline, FMOC-OH, methionine, valine, phenylalanine, leucine, histidine and lysine.
  • Analysis of Aflatoxins by HPLC
    Analysis of Aflatoxins, Grom Application Number 01075 A mixture of four aflatoxins was separated using a GROM-SIL 100 ODS-0 AB HPLC column (125 x 4mm, Part No. GSOD00510S1204) by isocratic elution with acetic acid, methanol, water 2/2/2008 in around 6 minutes and detected by fluorescence spectroscopy at 360nm. The compounds separated were G2a, B2a, G2 and B2.
  • Determination of Beta Blockers in Human Plasma
    Determination of B-Blockers in Human Plasma, Grom Application Number 01073 A mixture of two beta blocker drugs in human plasma (N-hydroxymethylatenolol and atenolol) was separated using a GROM-SIL 100 ODS-2 FE column (125 x 4mm, Part No. GSOD20510S1204) by isocratic elution with 50mM potassium phosphate, pH 2.2 and acetonitrile 81/19 in around 6 minutes and detected by fluorescence spectroscopy at 265 and 295nm.
  • Determination of D- and L-Amino Acids after Precolumn Derivatiza
    Determination of the D- and L- Amino Acids after Precolumn Derivatisation with OPA/N-Isobutryl-L-Cysteine, Grom Application Number 10064 The D- and L- forms of sixteen amino acids were separated using a GROM-SIL OPA-2 column (250 x 4mm, Part No. GSOP20512S2504) after precolumn derivatization with OPA/n-isobutryl-L-cysteine by gradient elution with 23mM sodium acetate, pH 6.0 and 600mL methanol and 50 mL acetonitrile in around 75 minutes and detected by fluorescence spectroscopy at 230 and 445nm. The amino acids separated were L-aspartic acid, D-aspartic acid, L-glutamic acid, D-glutamic acid, L-serine, D-serine, L-threonine, glycine, D-threonine, L-histidine, D-histidine, L-alanine, L-arginine, D-alanine, L-tyrosine, D-tyrosine, L-valine, L-methionine, L-nor-valine, D-methionine, D-valine, L-phenylalanine, D-isoleucine, D-phenylalanine, L-leucine, D-isoleucine, D-leucine, L-lysine and D-lysine.
  • Determination of Propanolol-Diastereomers of Beta-Blocker
    Determination of Propanolol-Analysis of Diastereomers of Beta-Blockers in a Pill, Grom Application Number 10053A The diastereomers of propanolol, (L-propanolol and D-propanolol) a Beta blocker compound were separated isocratically using a GROM-SIL 100 ODS-2 FE column (125 x 4mm, Part No. GSOD20510S1204) by isocratic elution with water and acetonitrile 30/70 in around 10 minutes and detected by fluorescence at 263 and 313nm.
  • High Sensitivity Amino Acid Analysis of Protein Hydrolysate
    High Sensitivity Amino Acid Analysis of Protein Hydrolysate by Microbore HPLC after Precolumn Derivatisation with OPA, Grom Application Number 10063 Fifteen amino acids in a protein hydrolysate were separated using a GROM-SIL OPA-1 microbore column (250 x 4mm, Part No. GSOP10508S2501) after precolumn derivatization with OPA by gradient elution with 12.5mM sodium phosphate, pH 7.2, acetonitrile and methanol 97/0.5/2.5 and 12.5mM sodium phosphate, pH 7.2, acetonitrile and methanol 70/25/5 in around 26 minutes and detected by fluorescence spectroscopy at 330 and 450nm. The amino acids separated were aspartic acid, glutamic acid, serine, glycine, threonine, histidine, alanine, arginine, tyrosine, valine, methionine, ammonia, isoleucine, phenylalanine, leucine and lysine.
  • Optical Antipodes of a Beta-Blocker by HPLC
    High Speed HPLC Analysis Determination of Optical Antipodes of a B-Blocker, Grom Application Number 10053b A mixture of two beta-blocker enantiomers was separated using a GROM-SIL 100 ODS-2 FE column (33 x 4.6mm, Part No. GSOD20210S0405) by isocratic elution with water and acetonitrile 30/70 in around 3 minutes and detected by fluorescence spectroscopy at 263 and 313nm. The compounds separated were D-propanolol and L-propanolol.
  • Polyamines in Decaying Fish by FMOC/EVE Derivatization
    Determination of Polyamines in Decaying Fish Fillet Applying Precolumn Derivatisation with FMOC/EVE and HPLC, Grom Application Number 10059 Five polyamines in a decaying fish fillet were separated using a GROM-SIL Polyamine-1 column (200 x 4mm, Part No. GSPO10510S2004) by gradient elution with 50 mM sodium acetate, pH 4.0 and acetonitrile 80/20 and 50 mM sodium acetate, pH 4.0 and acetonitrile = Mai 95 in around 15 minutes and detected by fluorescence spectroscopy at 263 and 310nm. The polyamines separated were putrescine, cataverine, histamine, spermidine and spermine.
  • Polyamines in Fresh and Spoiled Meat by FMOC/EVE Derivatization
    Analysis of Polyamines in Fresh and inSpoiled Meat (Pork) by Precolumn Derivatisation with FMOC/EVE, Grom Application Number 10060 Five polyamines in fresh and spoiled meat were separated using a GROM-SIL Polyamine-1 column (200 x 4mm, Part No. GSPO10510S2004) by gradient elution with 50 mM sodium acetate, pH 4.0 and acetonitrile 80/20 and 50 mM sodium acetate, pH 4.0 and acetonitrile = Mai 95 in around 15 minutes and detected by fluorescence spectroscopy at 263 and 310nm. The polyamines separated were putrescine, cataverine, histamine, spermidine and spermine.
  • Polyamines in Meat by FMOC/EVE Derivatization
    Analysis of Polyamines in Fresh and in Meat Employing the FMOC/EVE Approach, Grom Application Number 10103 Seven polyamines in a meat extract were separated using a GROM-SIL Polyamine-2 column (250 x 4mm, Part No. GSPO20510S2504) by gradient elution with water, acetic acid and acetonitrile 400/3/100 and water, acetic acid and acetonitrile 25/3/475 in around 4.5 minutes and detected by fluorescence spectroscopy at 263 and 310nm. The polyamines separated were phenylmethylamine, putrescine, cadaverine, tyramine, histamine, spermidine and spermine.
  • Primary and Secondary Amino Acids by HPLC
    Primary and Secondary Amino Acids, Grom Application Number 10165 A mixture of 24 amino acids was separated using a GROM-SIL OPA-2 HPLC column (250 x 4mm, Part No. GSOP30309S1204) by gradient elution with 23mM sodium acetate, pH 6.0 and 600 mL methanol and 50 mL acetonitrile in around 26 minutes and detected by fluorescence spectroscopy at 230 and 445nm.
  • Protein Hydrolysate Standard
    Protein Hydrolysate Standard (6N HCl/15 hours), Grom Application Number 10054 Amino acids in a protein hydrolysate standard (6N HCl/15 hours) were separated using a GROM-SIL FMOC-2 column (250 x 4mm, Part No. GSFM20512S2504) by gradient elution with 50 mM sodium acetate and acetonitrile 80/20 and 50 mM sodium acetate/acetonitrile 20/80 at pH 4.2 in around 50 minutes and detected by fluorescence at 263 and 313nm. The amino acids and other compounds separated were cysteine sulphinic acid, arginine, serine, aspartic acid, glutamic acid, threonine, glycine, alanine, tyrosine, proline, FMOC-OH, ammonia, methionine, valine, nor-valine, phenylalanine, isoleucine, leucine, histidine and lysine.
  • Separation of Chemotherapeutic Rubicins by HPLC
    Rubicines - Chemotherapeutics, Grom Application Number 01029 Two chemotherapeutic rubicins in human plasma were separated using a GROM-SIL 120 Octyl-5 CP column (125 x 4mm, Part No. GSOD30512S1203) by gradient elution with 25mM sodium phosphate, pH 2.2 and acetonitrile in around 12 minutes and detected by fluorescence spectrometry at 480 and 560nm. The rubicins separated were doxorubicin and daunorubicin.
  • Separation of D, L Amino Acid Standard
    Separation of a D, L Amino Acid Standard by HPLC Employing the FLEC-ADAM Approach, Grom Application Number 10058a A mixture of 16 amino acids was separated into their D and L stereoisomers using a GROM-SIL FLEC-1 column (250 x 4mm, Part No. GSFL10312S2504) by gradient elution with 50mM sodium acetate, pH 4.0 and acetonitrile in around 72 minutes and detected by fluorescence spectroscopy at 263 and 313nm. The amino acids separated were D-arginine, L-arginine, D-serine, L-serine, D-aspartic acid, L-aspartic acid, D-threonine, L-threonine, D-glutamic acid, L-glutamic acid, D,L-proline, D-alanine, L-alanine, D-tyrosine, L-tyrosine, D-methionine, L-methionine, D-valine, L-valine, D-phenylalanine, L-phenylalanine, D-alloisoleucine, L-alloisoleucine and L-isoleucine, D-leucine, D-histidine, L-histidine, D-ornithine, L-ornithine, D-lysine, and L-lysine.
  • Separation of D, L Proline After Derivatization
    Isocractical Separation of D- and L-Proline After Precolumn Derivatisation with FLEC-ADAM, Grom Application Number 10058b The amino acid proline was separated into its D and L stereoisomers using a GROM-SIL FLEC-1 column (250 x 4mm, Part No. GSFL10312S2504) by isocratic elution with 50mM sodium acetate, pH 4.0 and acetonitrile 55/45 in around 20 minutes and detected by fluorescence spectroscopy at 263 and 313nm.