HPLC separation with electrochemical detection.
- Separation of Seven Catecholamines by HPLC
Catecholamines II, Grom Application Number 01027
A mixture of seven catecholamines was separated using a GROM-SIL 120 ODS-4 HE column (150 x 4mm, Part No. GSOD40512S2504) by isocratic elution with a mixture of 6.9g sodium phosphate, 37mg EDTA, 150mg sodium octane sulfonate, 60mL acetonitrile, 5mL of THF and the rest made up to 1000mL with water in around 20 minutes and detected by electrochemical detection. The catecholamines separated were noradrenaline, 3,4-dihydroxyphenylacetic acid, 3,4-dihydroxyphenylalanine, dopamine, epinine, homovanillic acid and 3-O-methyldopa.
- Separation of Two Neuroleptics by HPLC
Neuroleptics, Grom Application Number 06034
Two neuroleptic compounds in rabbit plasma were separated using a GROM-SIL 120 CN-3 CP column (50 x 4mm, Part No. GSCN30512S) by isocratic elution with 0.1M sodium acetate, pH 5.0 and acetonitrile 36/64 in around 15 minutes and detected by electrochemical detection at 850mV. The neuroleptic compounds separated were chlorpromazine and promazine.