Similarly, in the chromatograms shown on the right hand side of figure 3, 2, 4 and 6 ml of a solution each containing 9.0 mg of naphthalene was injected onto the column and. The maximum sample volume that just permits the separation of naphthalene and anthracene, identified experimentally was 6 ml (theoretical value calculated from equation 2 was 6.1 ml). Destefano and Beachel (4) has also investigated the effect of volume and mass overload on resolution. They concluded that, given the choice, it is advantageous to overload a column with a large volume of a dilute solution of sample, as an alternative to using a small volume containing a high concentration of sample. They reported, however, that the validity of this conclusion, appeared to depend somewhat on the capacity ratios of the eluting solutes. In figure 4 the retention distance (measured in cm along the chart) is plotted against the sample volume

After, J. Chromatogr., Ref. [3]

 

Figure 4. Retention Distance of Benzene, Naphthalene and Anthracene against Sample Volume