Plate Theory and Extensions - Vacancy Chromatography > Page 76


Let the volume of sample injected be (Vi) ml, then the charge measured in 'plate volumes' will be (). Similarly, if the volume of mobile phase passed through the column is (y) ml then that will be equivalent to  plate volumes. Assuming the sample that is injected onto the column contains solute (S) at a concentration Xs1, then from equation (25), the concentration of this solute () leaving the (n) th plate in the column will be given by:-


Thus, for a chromatogram of (q) solutes, the elution curve equation will be given by,

  .    (64)

If pure mobile phase is injected onto the column, then Xs= 0 and equation (26) becomes