Liquid Chromatography - The Tridet Multi Functional Detector 3


It is seen that the anthracene is clearly picked out from the mixture of aromatics by the fluorescence detector and the chloride ion, not shown at all by the UV adsorption or fluorescence detectors, is monitored by the electrical conductivity detector. The simultaneous use of all detector functions make this detector very useful but, the real advantage of the trifunctional detector is that it allows the analyst a choice of the three most useful detector function in one detecting system. In addition, any of the three functions can be chosen at the touch of a switch and without any changes in hardware. An example of the use of the three individual detector function in the analysis of three quite different types of sample demonstrates this advantage.


Column Pecosphere

Column Pecosphere 3

Size 3 mm x 3 cm C18

Size 4.6 mm x 15 cm C18

Size 3 mm x 3 cm C18

17% Methanol/Water

90% Acetonitrile/Water

1nMtetrabutyl-ammonium hydroxide and buffer

Flow-Rate 3.0 ml/min.

Flow-Rate 2 ml/min.

1.5 ml/min.

UV Detector

Fluorescence Detector

Conductivity Detector


Sample Composition


1 Theobromine

1 Naphthalene

1 Solvent peak

2 Theophyline

2 Fluorene

2 Chloride ions

3 hydroxyethyltheophyline

3 Acenaphthene

3 Nitrite ions

4 Caffeine

4 Phenanthrene

4 Bromide ions


5 Anthracene

5 Nitrate ions


6 Fluoranthene

6 Phosphate ions


7 pyrene

7 Phosphite ions


8 Benzo(a)anthracene

8 Sulfate ions


9 Chrysene

9 Iodide ions


10 Benzo(b) Fluoranthene



11 Benzo(k) Fluoranthene



12 Benzo(k) Fluorantmene



13 Dibenz(a,h)anthracene

14 Idenol(1,2,3-cd))pyrene



15 Benzo(ghi)perylene



Figure 28. Chromatograms from the Trifunctional Detector