The Mechanism of Chromatographic Retention - Solute Stationary Phase Interactions > Experimental Support for the Sorption and Displacement Process > Page 56

The two solutes, anisole and nitrobenzene, are not well retained by the same phase system in a chromatographic column. In fact, on a silica gel column packed with the same silica and chromatographed with the same solvent mixture, anisole and nitrobenzene are eluted at capacity ratio (k') values of 2.4 and 4.7 respectively.


Initial Concentration of Ethyl AcetateinMobile Phase 0.35%w/v.

Volume of Mobile Phase 100 ml. Mass of Silica 10 gram.

Em = Mass of Ethyl Acetate in Mobile Phase Sm = Mass of Solute in Mobile Phase.

ES = Mass of Ethyl Acetate on Silica Gel SS = Mass of Solute on the Silica Gel


Figure 26. Graphs of Mass of Solute and Mass of Polar Solvent Contained in the Mobile Phase and on the Silica Gel Respectively against Mass of Solute Added