The Thermodynamics of Chromatography - The Distribution of Standard Energy Between Different Types of Molecular Interactions > The Concept of Complex Formation > Page 51

The fraction (c) of the complexed solute is given as,

                or 

Substituting for (1-c) in the equation for (),

Unfortunately, it is impossible to separate the terms () and  and, thus, the procedure must be complicated further by the introduction of four additional specific retention volumes. Experimental values for these can be used (with certain assumptions)

to obtain a value for (K') and consequently a value for ().