Extra Column Dispersion - Separations on Small Bore Columns 2m Long > Page 67
Unfortunately, if you have a mixture that requires very high efficiencies and an analysis time of several hours is considered too long, the only alternative is to seek a different sample!!
The separation of a mixture of aromatic hydrocarbons on a 2 m column is shown in figure 36.
Column Length 2 m, column I.D. 1 mm, packing ODS 2 reversed phase, 10 mm in diameter, mobile phase 75% v/v acetonitrile in water, flow rate 38 ml/ min., sample volume 0.5 ml, efficiency 160,000 theoretical plates
Figure35. ChromatogramofaComplexMixtureof Aromatic Hydrocarbons Separated on a Small Bore Column 2 M Long