Extra Column Dispersion - Dispersion Due to Detector Electronics Response Time > Page 49

 

The quantitative evaluation of seven duplicate analyses is shown in Table 5.

 

Table 5. Reproducibility of the Quantitative Analysis by Peak Area Normalization of a Five Component Synthetic Mixture (Isocratic High Speed Separation)

Compound Normalized Peak Area Mean Mean s s %
p-Xylene 9.8 9.8 9.6 9.8 9.8 9.6 9.7 0.10 1.1  
Anisole 11.9 11.9 12.2 12.2 11.9 12.0 12.0 0.17 1.4  
Nitrobenzene 24.2 23.9 24.7 24.2 24.0 24.3 24.2 0.28 1.2  
Acetophenone 23.7 23.9 23.5 23.4 23.3 23.7 23.6 0.22 1.0  
DiProPhthalat 30.3 30.5 30.0 30.4 31.1 30.4 30.5 0.36 1.2  

It is seen that providing the chromatograph is well designed, and appropriate attention paid to extra column dispersion, in particular to the peed of response of the detecting system, then very accurate analyses can be carried out even with analysis time of less the 4 seconds.