Plate Theory and Extensions - Resolving Power of a Column > Page 60

 

where (KA) is the distribution coefficient of the first eluted solute between the two phases.

 

Figure 17. Chromatogram Showing Two Resolved Peaks

 

Thus, if resolution is achieved when the peak maxima of the pair of solutes are (4s) apart, then

                             

        Rearranging,                            

 Dividing through by (vm), and noting that ,

                                                     

 The separation ratio, (a), between the two solutes, has been defined as