Liquid Chromatography Detectors - LC Detectors Based on Refractive Index Measurement > The Angle of Deviation Method > Page 22

Figure 11. The Refractive Index Detector Based on the Angle of Deviation Method of Measurement

 

In fact, it is the location of the beam, rather than its intensity, that changes with the refractive index difference between the contents of the two cells. As the position of focus of the beam on the photoelectric cell changes, the electrical output changes which is electronically modified to provide a signal proportional to the concentration of solute in the cell.

Figure 12. Chromatogram from an RI Detector Based on the Angle of Deviation Method of Measurement

An example of a separation monitored by a refractive index (RI) detector for a typical sugar application  is shown in figure 12.