Preparative Chromatography - Recycling Development > Page 43

The chromatograms in figure 22 show that each fraction is very pure with very little isomer contamination. This relatively high purity was obtained, using a 400 mg charge, in less than 25 minutes. It is clear that the recycling procedure, coupled with the peak shaving technique, can handle significant column loads, provide the required resolution and at the same time provide excellent solvent economy.

 

Courtesy of ASTEC Inc.

 

Figure 22. Chromatograms of the 5-Methyl-5-Phenyl-Hydantoin Enantiomer Fractions