Preparative Chromatography - Radial Flow Chromatography > Page 64

The method used for packing radial columns is depicted in figure 33. The packing is prepared in the form of a slurry and is pumped directly into the column between the two frits. The column exit to the detector and fraction collector is closed, and, as a result, the slurry solvent passes through the outer frit and exits via the normal mobile phase inlet port. The columns are very easily unpacked by adopting the reverse procedure.

Figure 34. The Separation of Some Large Biomolecules Using Radial-Flow Reverse Phase Chromatography