Plate Theory and Extensions - Quantitative Analysis from Retention Measurements > Page 51

             (45)

where (C) is a constant,
  (a) is the response of the detector to solute (A),
and (b) is the response of the detector to solute (B).

Equation (45) was examined by Scott and Reese [12] using mixtures of nitrobenzene and fully deuterated nitrobenzene as the test sample. The retention times of the solutes were 8.927 min. and 9.061 min., respectively, having a difference of 8.04 seconds. The separation ratio of the two solutes was 1.023 and the efficiencies of the front and rear portions of the peaks were 5908 and 3670 theoretical plates, respectively. The detector was found to have the same response to both solutes, i.e., a = b. Thus, inserting these values in equation (45),