Preparative Chromatography - Packing Preparative Columns > GC Columns > Page 31

GC Columns

Small GC columns, up to 2 or 3 ft long and up to 0.5 in. in diameter can be hand packed using techniques similar to those used in the preparation of analytical columns. The system used is shown on the right hand side of figure 14. The column is fitted with a pre-column so that the column proper can be packed 3 or 4 inches longer than is actually required. The column is packed in a vertical position by adding small quantities of packing (about 2 ml) at a time and tapping the sides of the column until the bed settles to a minimum volume. This procedure is continued until the system is completely packed.

Figure 14. Apparatus for Packing Preparative GC Columns