The Thermodynamics of Chromatography - Other Thermodynamic Methods that are Used for Studying Chromatographic Systems > Page 57

                                            (29)

where(a(1)(2)) is the separation ratio of solute (1) to solute (2),
(V'(1)) is the corrected retention volume of solute (1),
(V'(2)) is the corrected retention volume of solute (2),
(K(1)) is the distribution coefficient of solute (1) with respect to the stationary phase,
(K(1)) is the distribution coefficient of solute (2) with respect to the stationary phase,
and (VS) is the volume of stationary phase in the column.

The corrected retention volume is defined as the difference between the retention volume and the thermodynamic dead volume. Bearing in mind that the distribution coefficients given in equation (28) are the reciprocal of the distribution coefficients given in equation (29), then

                                     (30)

where the subscripts (1) and (2) refer to solutes (1) and (2) and the subscripts (A), (B) and (AB) have the meaning previously ascribed to them.