The Thermodynamics of Chromatography - Other Thermodynamic Methods that are Used for Studying Chromatographic Systems > Optimum Operating Conditions for Chiral Separations in Liquid Chromatography > Page 74

Substituting the data from tables 7 and 8 in equations (37) and (38), expressions for the capacity ratios of the two enantiomers [given by equations (47) and (48)] and their separation ratios [given by equation (49)] can be obtained which will permit these parameters to be calculated for any combination of temperature or solvent compositions. 

                   (47)

                           (48)

 

                    (49)

The capacity ratios and separation ratios obtained from equations (47), (48) and (49) can be employed to calculate the other parameters that influence the separation, (e.g. column length and analysis time) at different temperatures and solvent compositions. Moreover, it will be possible to identify the best practical column length and temperature, that will give the minimum analysis time.