The Thermodynamics of Chromatography - Other Thermodynamic Methods that are Used for Studying Chromatographic Systems > Optimum Operating Conditions for Chiral Separations in Liquid Chromatography > Page 68

   

 

The corrected retention volumes of each enantiomer are shown plotted against the reciprocal of the volume fraction of ethanol, for each temperature mixture in  figures 16A and 16B. It is seen that the data fits the reciprocal function extremely well with indices of determination very close to unity. The slopes and intercepts of the linear curves shown in figures 16A and 16B are summarized in table 6.

Table 6 Slopes and Intercepts from Curves Relating the Corrected Retention Volume of the (S)- and (R)- Enantiomers of 4-Benzyl-2-Oxazolidinone against the Reciprocal of the Volume Fraction  of Ethanol in the Mobile Phase.

 

Ethanol v/v d(V'S)/d(1/c) d(VR)/d(1/c) Intercept (S) Intercept (R)
10 % 6.778 5.505 5.316 4.673
20 % 5.486 4.639 4.387 3.989
30 % 4.409 3.779 3.404 3.230
40 % 3.806 3.350 3.234 2.987
50 % 3.174 2.820 2.719 2.285