The Thermodynamics of Chromatography - Other Thermodynamic Methods that are Used for Studying Chromatographic Systems > Optimum Operating Conditions for Chiral Separations in Liquid Chromatography > Page 65

                                    

 

Assuming a practical value of 2 x 10-5 cm2 s for (Dm) and 5 x 10-4 cm for (dp) (5 micron particles)

                                                 (43)

Thus, the minimum analysis time (tmin.) will be,

                                                                                               (44)

  

Thus, if it is possible to identify fa(T,c) and fb(T,c) by experiment, then employing equations (42) and (44), the column length and the minimum analysis time can be calculated for any combination of (T) and (c). Consequently, by the use of a simple computer program, the practical range of (T) and (c) can be examined, and the optimum temperature (T), and the optimum mobile phase composition (c), (should they exist) that gives the minimum analysis time can be identified.