The Thermodynamics of Chromatography - Other Thermodynamic Methods that are Used for Studying Chromatographic Systems > Optimum Operating Conditions for Chiral Separations in Liquid Chromatography > Page 64

Now, (Hmin.) (the minimum variance per unit length of the eluted peak) to the first approximation, has been shown (see book 9 of this series) to be,


where (dp) is the particle diameter of the packing.

The length (L) of the column is ,

                                 L = nHmin.                                                             (41)

Substituting for (n) from (39) and for (Hmin.) from (40) in (41),



Similarly, it has been shown (15), the optimum mobile phase velocity is given by,