The Thermodynamics of Chromatography - Other Thermodynamic Methods that are Used for Studying Chromatographic Systems > Optimum Operating Conditions for Chiral Separations in Liquid Chromatography > Page 64
Now, (Hmin.) (the minimum variance per unit length of the eluted peak) to the first approximation, has been shown (see book 9 of this series) to be,
where (dp) is the particle diameter of the packing.
The length (L) of the column is ,
L = nHmin. (41)
Substituting for (n) from (39) and for (Hmin.) from (40) in (41),
Similarly, it has been shown (15), the optimum mobile phase velocity is given by,