The Mechanism of Chromatographic Retention - Mixed Phases > Page 27

In the experiments of Katz et al., the distribution coefficients (K) referred to the solvent phase (which would be the mobile phase in normal LC), whereas in LC it is the mobile phase composition that is changed and the distribution coefficients (K") would then be referred to the stationary phase (water and not the solvent mixture).

Thus,

or,

Substituting for the corrected retention volumes in equation (1) for inverse phase system,

Simplifying, (3)

when,

An alternative way of expressing solute retention in terms of solvent concentration for a binary solvent mixture as the mobile phase is to use the inverse of equation (3), i.e.,