Extra Column Dispersion - Dispersion in Contemporary LC Columns > Page 4

Dispersion in Contemporary LC Columns

The standard deviation volume of an eluted peak (sv) is given by, (see Plate Theory and Extensions),

where, (Vr) is the retention volume of the solute and (n) is the column efficiency.

Now, Vr = Vo(1+k') = elpr2(1+k')

where (Vo) is the thermodynamic dead volume of the column, (i.e.,Vo=elpr2(1+k'))
(r) is the radius of the column,
(l) is the length of the column,
and (e) is the fraction of the mobile phase in the column that is available to the solute

Consequently,

Now

Now, for a well packed column H = 1.6 dp, where (dp) is the particle diameter of the packing (1)