Liquid Chromatography Detectors - The Fluorescence Detector > The Multi Wavelength Fluorescence Detector > Page 61

Optimizing excitation and emission light wavelengths to obtain maximum sensitivity for a complex mixture can become quite involved as shown by the  separation of some priority pollutants depicted in figure 40. The separation was carried out on a column 25 cm long, 4.6 mm in diameter and packed with a C18 reversed phase. The mobile phase was programmed from a 93% acetonitrile, 7% water to 99% acetonitrile, 1% water over a period of 30 minutes. The gradient was linear and the flow rate was 1.3 ml/min.

Courtesy of the Perkin Elmer Corporation

 

1 Naphthalene   9 Chrysene
2 Acenaphthene 10 Benzo(b)fluoranthene
3 Fluorene 11 Benzo(k)fluoranthene
4 Phenanthrene 12 Benzo(a)pyrene
5 Anthracene 13 Dibenz (a,h)anthracene
6 Fluoranthene 14 Benzo(ghi)perylene
7 Pyrene 15 Indeno(123-cd)pyrene
8 Benz(a)anthracene