Liquid Chromatography Detectors - Dispersion in Detector Sensors > Dispersion in the Detector Sensor Volume > Dispersion in Detector Sensors Resulting from Newtonian Flow > Page 11

 

Figure 5 Elution Curves Presented as a Function of the Normalized Tube Length

 

It is seen that serious peak distortion can occur but as the length of the sensor cell is increased the distortion is reduced, but the dispersion increases. However, if the conduits to the cell are appropriately designed to produce secondary flow in the cell, then the parabolic velocity profile is destroyed, and the dispersion and peak distortion eliminated. The manner of entry of the mobile phase from the connecting conduits are, consequently, designed to produce this secondary flow and the manner in which this is achieved is shown in figure 6.