Liquid Chromatography - Macrocyclic Glycopeptide Phases 5


The column used was 25 cm long, 4.6 mm I.D., packed with Chirobiotic T. The mobile phase was methanol containing acetic acid and triethylamine in the concentrations shown in figure 52. The column was operated at room temperature and at a flow rate of 2 ml/min. Teicoplanin is stable over a pH range of 3.8 to 6.5 although it can be used for limited periods of time outside this range. By suitable choice of mobile phase, Teicoplanin can be used in the reversed phase mode.

Another macrolytic glycopeptide that has been used as a chiral stationary phase, is the glycopeptide, Avoparcin [40]. This stationary phase is available as CHIROBIOTIC A. Avoparcin is an antibiotic complex produced by Streptomysces candidus. In particular, it is used to prevent necrotic enteritus in chickens. The structure of avoparcin is shown in figure 53.

Figure 53. The Structure of Avoparcin