Liquid Chromatography - Cyclodextrin 3
The cyclodextrins have found a wide field of application in chiral chromatography and there arc many applications in the literature The following is a simple example of the use of this stationary phase.
In order to carry out in vivo pharmacological profiling of enantiomeric drugs, the direct analysis of biological fluids is required to reduce sample preparation time, and the chance of enantiomeric change. Unfortunately, many chiral stationary phases, such as the Pirkle types phases and the derivatized cellulose phases, demand the use of mobile phases that are incompatible with the biological fluids. Haginaka and Wakai  suggested that the silica should first be reacted with (3-glycidoxypropyl)trimethoxysilane, to cover a significant part of the silica surface with 'spacers', and then the remaining silanol groups reacted with cyclodextrin–carbamoylated triethoxysilane to attach the chiral agent.
Courtesy of J. Liq. Chromatogr. [Ref.17]
Figure 56. The Separation of the Hexobarbital Enantiomers Contained in Blood Serum by Direct Injection on a Cyclobond Phase