Plate Theory and Extensions - The Capacity Ratio of a Solute > Page 16
The Capacity Ratio of a Solute
As (K) is a unique property of a given solute its corrected retention volume, which is calculated from its corrected retention time is used as a means of identification. Thus the precision and accuracy of measurement depended on the precision and accuracy of the flow rate measurement. To avoid errors associated with flow rate measurement, the capacity ratio of a solute (k') was introduced.
The capacity ratio of a solute is defined as the ratio of its distribution coefficient to the phase ratio (a) of the column, where
Thus, and, as V'r = KVS
Then In practice, (21)
The value of (k') from experimental data, if not calculated by the data processing computer, is measured from the chart as,