Liquid Chromatography - Bonded Phases

Bonded Phases

Bonded phases are formed by reacting the surface hydroxyl groups with an appropriate reagent to chemical link an organic moiety to the silica surface. The nature of the organic moiety will determine the type of interaction that will take place between the solute and the surface. If the moiety is a hydrocarbon chain, then the interaction will be dispersive, if, for example, it contains a cyano group, it will be polar and if it carries a group that can dissociate into ions, the interactions will be ionic.



Figure 34. Graph of Calculated Weight Loss/Temperature

for Each Desorption Stage