Liquid Chromatography - The Retention Properties of Bulk and Brush Phases 1

The Retention Properties of Bulk and Brush Phases.

The equation for the corrected retention volume of a solute (V'r ) (see Plate Theory and Extensions ) is as follows,

V'r = KVS  or V'r = KAS

where (K) is the distribution coefficient of the solute between the two


(VS) is the effective volume of stationary phase in the column,

and (AS) is the effective surface area of the packing.

The two equations are given to illustrate that the reverse phase system may be considered as a liquid/liquid or liquid/solid distribution system where,

VS = dfAS

and (df) is the effective film thickness of the bonded material and

any solvent that may be adsorbed on its surface