# Liquid Chromatography - The Retention Properties of Bulk and Brush Phases 1

## The Retention Properties of Bulk and Brush Phases.

The equation for the corrected retention volume of a solute (V'_{r }) (see Plate Theory and Extensions ) is as follows,

V'_{r } = KV_{S} or V'_{r} = KA_{S}

where (K) is the distribution coefficient of the solute between the two

phases,

(V_{S}) is the effective volume of stationary phase in the column,

and (A_{S}) is the effective surface area of the packing.

The two equations are given to illustrate that the reverse phase system may be considered as a liquid/liquid or liquid/solid distribution system where,

V_{S} = d_{f}A_{S}

and (d_{f}) is the effective film thickness of the bonded material and

any solvent that may be adsorbed on its surface