Thin Layer Chromatography - An MS Hybrid Thin Layer Plate Transport Interface for Peptide-Like Materials 1

 

A Mass Spectrometric Measurement of Peptide-Like Materials Using a Hybrid Thin Layer Plate as the Transport Interface to a Matrix Assisted Laser Desorption Ionization System (15)

 

A hybrid TLC plate system has been employed as a transport medium in a novel manner to monitor a thin layer separation by means of a mass spectrometer. This unique combination has been employed to assay substances of biological origin at exceptionally low levels of concentration. The essential transport medium consisted of a thin layer plate, half of which was coated in the usual manner and on which the separation was developed. The other half of the plate consisted of a special coating that formed the base of the so called matrix assisted laser desorption ionization source for the mass spectrometer. A diagram of the hybrid plate is shown in figure 26.

 

 

Figure 26. The Hybrid Thin Layer Plate that Acts as a Transport Interface to a Matrix Assisted Laser Desorption Ionization Source for a Mass Spectrometer