Principles and Practice of Chromatography - Chromatography Applications > Liquid Chromatography Applications > Page 81
Courtesy of Supelco Inc.
Figure 42. Chromatograms of Tetrahydrocannabinol Car-boxylic Acid from Urine
The chromatograms obtained from a urine sample and a reference standard by this procedure is shown in figure 42. This is a typical application for liquid chromatography using a solid phase extraction cartridges. It is seen that the tetrahydrocannabinol carboxylic acid is clearly and unambiguously separated from the contaminating materials with an extraction efficiency of over 90%. The chromatographic conditions were as follows.
|Column Length||25 cm|
|Column Diameter||4.6 mm|
|Column Packing||C18 Reverse Phase, (octadecyldimethyl chain)|
|Mobile Phase||55% acetonitrile 45% aqueous 1% acetic acid|
|Detector||UV adsorption at 280 nm|
|Sample Volume||100ml containing 500 ng tetrahydrocannabinol|