Capillary Chromatography - Carborane Based Stationary Phases

Carborane Based Stationary Phases


An example of the use of a carborane phase at high temperature to separate the components of POLYWAX 1000 using a wide ranged temperature program shown in figure 37.



Courtesy of SGE Ltd.


Figure 10. The GC Analysis of POLYWAX 1000


The stationary phase used was the SGE HT5, a carborane modified siloxane stationary phase carrying 5 % phenyl groups. capable of operating at 480ûC. The column (aluminum clad as opposed to imide coated) was 12 m long, 0.53 mm I.D. and carried a film of stationary phase 0.15 mm thick. Initially, the column temperature was 200 ûC which was then raised to 480ûC at a rate of 10ûC/min.. A split injector was used to place the sample on the column. Hydrogen was used as the carrier gas at an inlet pressure of 5 psi. and the detector employed was an FID.




The solutes separated ranged from C30 to about C100 and the column bleed at 480ûC, although significant, was not excessive. The chromatogram could be used for accurate quantitative analysis providing suitable data acquisition and processing software was available. It is also significant that the separation was completed in under 35 minutes.