Thin Layer Chromatography - Forced-Flow Development
An additional technique of forced-flow plate development is even closer to normal LC techniques and virtually turns the thin layer plate into an actual lamina LC column.
The apparatus used for forced flow thin layer chromatography is shown in figure 9. The TLC plate is sandwiched between two metal plates that are sealed by appropriate gaskets. The upper plate is fitted with three conduits. The first conduit supplies mobile phase to one end of the plate; the second conduit is used to place the sample onto the plate at a position a little in advance of the solvent supply conduit; at the other end of the plate there is the third conduit that allows the solvent to leave the chromatographic system. The forced-flow development arrangement in the single dimensional mode appears a little illogical as a normal LC column system would appear to provide a far more efficient and simpler alternative. If the forced flow system is employed in a two dimensional manner, however, then the situation is quite different and a system is provided that cannot be simply emulated by normal LC systems.