Preparative Chromatography - Alternative Preparative Techniques > The Simulated Moving Bed Preparative Chromatography System > Page 55

The Simulated Moving Bed Preparative Chromatography System

The physical system described above was found to be extremely tricky to operate (although with modern computer control technology, the difficulties may well be significantly reduced) and this stimulated research into alternative moving bed systems. In 1971, Barker (13) and in 1973 Barker and Deeble (14) used a column in circular form to imitate the falling bed system. A diagram representing the wheel concept of Barker and Deeble is shown in figure 28.

The actual column consisted of a peripheral groove in a large wheel in which the packing is placed and contained by a plate placed on the outside after packing. The wheel somewhat resembled the driving wheel of a medium sized steam locomotive.

The ports to the column are fixed in positions at the periphery of the large wheel and the wheel had openings fitted with 'pop-valves regularly round the circumference to allow the ports to be continually connected to the peripheral column as it was rotated. The peripheral apertures were designed so that they were normally closed except when they coincided with the ports during rotation.


Figure 28 The Wheel Concept of Barker